Epidermal and dermal integration into sphere-templated porous poly(2-hydroxyethyl methacrylate) implants in mice.
Epidermal and dermal integration into sphere-templated porous poly(2-hydroxyethyl methacrylate) implants in mice.
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DOI：
10.1002/jbm.a.32798
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发表时间：
2010-09-15
影响因子：
4.9
通讯作者：
Fleckman, P.
中科院分区：
文献类型：
--
作者：
Fukano, Y.;Usui, M. L.;Underwood, R. A.;Isenhath, S.;Marshall, A. J.;Hauch, K. D.;Ratner, B. D.;Olerud, J. E.;Fleckman, P.
经皮医疗器械仍然容易受到感染和故障。我们假设皮肤愈合到经皮装置中将提供密封，防止细菌附着、生物膜形成和随后的装置故障。将具有球形模板孔（40μm）和互连喉部（16μm）的多孔聚（甲基丙烯酸2-羟乙酯）[聚（HEMA）]植入正常C57BL/6小鼠体内7、14和28天。 Poly(HEMA) 要么未经处理，保持表面对细胞和蛋白质不粘附，要么用羰基二咪唑 (CDI) 修饰或 CDI 与层粘连蛋白 332 反应以增强粘附。没有观察到感染的临床症状。使用光学和透射电子显微镜评估聚（HEMA）孔内的表皮和真皮反应。所有植入物的聚 (HEMA) 孔内都可以证实细胞（角质形成细胞、成纤维细胞、内皮细胞、炎症细胞）和基底膜蛋白（层粘连蛋白 332、β4 整合素、VII 型胶原）。在所有 14 天和 28 天的植入物中，血管和真皮胶原束都很明显。没有观察到纤维囊的形成和迁移。具有 40μm 孔径的球形模板聚合物表现出重现皮肤真皮层和表皮层关键元素的能力。我们的形态学研究结果表明，植入物模型可用于研究生物材料孔径、孔互连（喉部）尺寸和表面处理对皮肤生物整合的影响。此外，该模型可用于细菌挑战研究。
Percutaneous medical devices remain susceptible to infection and failure. We hypothesize that healing of the skin into the percutaneous device will provide a seal preventing bacterial attachment, biofilm formation, and subsequent device failure. Porous poly(2-hydroxyethyl methacrylate) [poly(HEMA)] with sphere-templated pores (40μm) and interconnecting throats (16μm) were implanted in normal C57BL/6 mice for 7, 14 and 28 days. Poly(HEMA) was either untreated, keeping the surface non-adhesive for cells and proteins, or modified with carbonyldiimidazole (CDI) or CDI reacted with laminin 332 to enhance adhesion. No clinical signs of infection were observed. Epidermal and dermal response within the poly(HEMA) pores was evaluated using light and transmission electron microscopy. Cells (keratinocytes, fibroblasts, endothelial cells, inflammatory cells) and basement membrane proteins (laminin 332, β4 integrin, type VII collagen) could be demonstrated within the poly(HEMA) pores of all implants. Blood vessels and dermal collagen bundles were evident in all of the 14 and 28 day implants. Fibrous capsule formation and permigration were not observed. Sphere-templated polymers with 40μm pores demonstrate an ability to recapitulate key elements of both the dermal and the epidermal layers of skin. Our morphological findings indicate that the implant model can be used to study the effects of biomaterial pore size, pore interconnect (throat) size, and surface treatments on cutaneous biointegration. Further, this model may be used for bacterial challenge studies.
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影响因子：
2.9
作者：
Fukano, Yuko;Knowles, Negar G.;Olerud, John E.
通讯作者：
Olerud, John E.
DOI：
10.1002/jbm.1253
发表时间：
2002-02-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Kidd, KR;Nagle, RB;Williams, SK
通讯作者：
Williams, SK
影响因子：
11.8
作者：
Crnich, CJ;Maki, DG
通讯作者：
Maki, DG
环氧树脂嵌入方法的改进。
DOI：
10.1083/jcb.9.2.409
发表时间：
1961-02
期刊：
The Journal of biophysical and biochemical cytology
影响因子：
--
作者：
LUFT JH
通讯作者：
LUFT JH
影响因子：
2.8
作者：
Chin, Curtis D.;Khanna, Krishn;Sia, Samuel K.
通讯作者：
Sia, Samuel K.