Potential role of transient receptor potential channel M5 in sensing putative pheromones in mouse olfactory sensory neurons.
Potential role of transient receptor potential channel M5 in sensing putative pheromones in mouse olfactory sensory neurons.
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DOI：
10.1371/journal.pone.0061990
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Restrepo D
中科院分区：
文献类型：
--
作者：
Oshimoto A;Wakabayashi Y;Garske A;Lopez R;Rolen S;Flowers M;Arevalo N;Restrepo D
基于对瞬时受体电位通道M5(Trpm5)基因敲除小鼠化学感觉转导的药理学研究，推测该通道参与了小鼠嗅觉感觉神经元(OSNs)信息素的转导。然而，在同一项研究中，小鼠嗅觉上皮的电嗅觉图(EOG)显示，野生型和Trpm5基因敲除小鼠对信息素(和气味)的反应没有显著差异。在这里，我们发现表达Trpm5的OSNs的数量在单侧鼻孔阻塞后增加。重要的是，EOG实验表明，缺乏Trpm5的小鼠对推测的信息素的闭塞上皮细胞的反应减弱，而野生型小鼠在单侧鼻孔闭塞后没有任何变化。这一证据表明，在嗅觉感觉输入减少的情况下，Trpm5在介导可能的信息素转导方面发挥了作用。此外，我们证明了环核苷酸门控通道A2基因敲除(CNGA2-KO)小鼠对气味和信息素的反应显著降低或缺失，与野生型小鼠相比，Trpm5水平也升高。综上所述，我们的证据表明，在化学感觉输入减少的条件下，Trpm5在介导推定信息素的转导方面发挥了作用。
Based on pharmacological studies of chemosensory transduction in transient receptor potential channel M5 (TRPM5) knockout mice it was hypothesized that this channel is involved in transduction for a subset of putative pheromones in mouse olfactory sensory neurons (OSNs). Yet, in the same study an electroolfactogram (EOG) in the mouse olfactory epithelium showed no significant difference in the responses to pheromones (and odors) between wild type and TRPM5 knockout mice. Here we show that the number of OSNs expressing TRPM5 is increased by unilateral naris occlusion. Importantly, EOG experiments show that mice lacking TRPM5 show a decreased response in the occluded epithelia to putative pheromones as opposed to wild type mice that show no change upon unilateral naris occlusion. This evidence indicates that under decreased olfactory sensory input TRPM5 plays a role in mediating putative pheromone transduction. Furthermore, we demonstrate that cyclic nucleotide gated channel A2 knockout (CNGA2-KO) mice that show substantially decreased or absent responses to odors and pheromones also have elevated levels of TRPM5 compared to wild type mice. Taken together, our evidence suggests that TRPM5 plays a role in mediating transduction for putative pheromones under conditions of reduced chemosensory input.
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DOI：
10.1186/1744-9081-6-26
发表时间：
2010-05-25
期刊：
Behavioral and brain functions : BBF
影响因子：
--
作者：
Angely CJ;Coppola DM
通讯作者：
Coppola DM
影响因子：
56.9
作者：
JONES, DT;REED, RR
通讯作者：
REED, RR
影响因子：
3.8
作者：
BRUNJES, PC
通讯作者：
BRUNJES, PC
DOI：
10.1016/0165-3806(91)90171-e
发表时间：
1991-10-21
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
BRUNJES, PC;CAGGIANO, AO;STEWART, JS
通讯作者：
STEWART, JS
影响因子：
3.8
作者：
Kimbell, JS;Godo, MN;Morgan, KT
通讯作者：
Morgan, KT