Genetic modification of alternative respiration in Nicotiana benthamiana affects basal and salicylic acid-induced resistance to potato virus X.
Genetic modification of alternative respiration in Nicotiana benthamiana affects basal and salicylic acid-induced resistance to potato virus X.
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DOI：
10.1186/1471-2229-11-41
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发表时间：
2011-02-28
影响因子：
5.3
通讯作者：
Carr JP
中科院分区：
文献类型：
--
作者：
Lee WS;Fu SF;Verchot-Lubicz J;Carr JP
水杨酸(SA)调节多种抗病毒机制，包括可能受线粒体酶负调控的机制(S)，交替氧化酶(AOX)，替代呼吸途径的唯一组成部分。然而，这种机制的研究可能会被SA介导的RNA依赖的RNA聚合酶1的诱导所混淆，RNA依赖的RNA聚合酶1是抗病毒RNA沉默途径的一个组成部分。我们获得了转基因烟草植株，在这些植株中，交替呼吸途径的能力要么通过AOX的结构性表达而增加，要么通过表达显性负突变蛋白(AOX-E)而降低。使用N.benthamiana是因为它是一个天然突变体，不表达功能依赖于RNA的RNA聚合酶1。抗霉素A(一种替代呼吸途径诱导剂，也是一种抗病诱导剂)和SA触发了对烟草花叶病毒(TMV)的抗性。AOX转基因植株对抗霉素A诱导的烟草花叶病毒的抗性受到抑制，而SA诱导的植株对烟草花叶病毒的抗性表现出较强的抗性。这些效应仅限于直接接种的叶片，不受构成表达功能性RNA依赖的RNA聚合酶(MtRDR1)的转基因的存在或缺失的影响。意想不到的是，感染马铃薯X病毒(PVX)的AOX转基因植株在接种和系统感染的叶片中显著增加了对系统疾病诱导和病毒积累的敏感性。在转AOX基因的植株中，SA诱导的对PVX的抗性减弱，而表达AOX-E的植株对该病毒的SA诱导的抗性增强。我们得出结论，AOX调控机制不仅在SA诱导的抗性中起作用，而且对某些病毒如PVX的基础抗性也有重要贡献。
Salicylic acid (SA) regulates multiple anti-viral mechanisms, including mechanism(s) that may be negatively regulated by the mitochondrial enzyme, alternative oxidase (AOX), the sole component of the alternative respiratory pathway. However, studies of this mechanism can be confounded by SA-mediated induction of RNA-dependent RNA polymerase 1, a component of the antiviral RNA silencing pathway. We made transgenic Nicotiana benthamiana plants in which alternative respiratory pathway capacity was either increased by constitutive expression of AOX, or decreased by expression of a dominant-negative mutant protein (AOX-E). N. benthamiana was used because it is a natural mutant that does not express a functional RNA-dependent RNA polymerase 1. Antimycin A (an alternative respiratory pathway inducer and also an inducer of resistance to viruses) and SA triggered resistance to tobacco mosaic virus (TMV). Resistance to TMV induced by antimycin A, but not by SA, was inhibited in Aox transgenic plants while SA-induced resistance to this virus appeared to be stronger in Aox-E transgenic plants. These effects, which were limited to directly inoculated leaves, were not affected by the presence or absence of a transgene constitutively expressing a functional RNA-dependent RNA polymerase (MtRDR1). Unexpectedly, Aox-transgenic plants infected with potato virus X (PVX) showed markedly increased susceptibility to systemic disease induction and virus accumulation in inoculated and systemically infected leaves. SA-induced resistance to PVX was compromised in Aox-transgenic plants but plants expressing AOX-E exhibited enhanced SA-induced resistance to this virus. We conclude that AOX-regulated mechanisms not only play a role in SA-induced resistance but also make an important contribution to basal resistance against certain viruses such as PVX.
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DOI：
10.1126/science.1171647
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boller T;He SY
通讯作者：
He SY
影响因子：
3.5
作者：
Ji, LH;Ding, SW
通讯作者：
Ding, SW
影响因子：
3.8
作者：
Lewsey, Mathew G.;Carr, John P.
通讯作者：
Carr, John P.
影响因子：
11.6
作者：
Chivasa, S;Murphy, AM;Carr, JP
通讯作者：
Carr, JP
DOI：
10.1007/bf00267408
发表时间：
1978-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
HOLSTERS, M;DEWAELE, D;SCHELL, J
通讯作者：
SCHELL, J