Time of death revealed by hydrocarbons of empty puparia of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae): a field experiment.

Time of death revealed by hydrocarbons of empty puparia of Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae): a field experiment.
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Chrysomya megacephala (Fabricius)(双翅目:Calliphoridae)空蛹碳氢化合物揭示的死亡时间:现场实验

DOI:
10.1371/journal.pone.0073043
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发表时间:
2013
期刊:
影响因子:
3.7
通讯作者:
Xu XH
Xu XH
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Zhu GH;Yu XJ;Xie LX;Luo H;Wang D;Lv JY;Xu XH

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死亡时间(PMI)的确定对于凶杀案的调查至关重要。然而,目前只有有限的方法可用。特别是,当PMI超过果蝇成虫羽化的成虫前发育期时,其测定结果非常近似。本文报道了食尸蝇中常见的一种大头蝇蛹在野外风化过程中烃类组成的规律性变化。相关分析表明,几乎所有的支链烷烃和烯烃的相对丰度随风化时间的延长而显著降低。特别是,对于9个峰,超过88%的变异,其丰度解释了风化时间。进一步分析表明,这些规律性的变化主要是由各种烃类的风化速率不同引起的。此外,风化率被发现取决于碳氢化合物的化学结构和分子量。这些结果强烈表明,碳氢化合物分析是一个强有力的工具,用于确定风化时间的尸食性蝇蛹,并预计将显着提高测定的后期PMI。
Determination of the postmortem interval (PMI) is crucial for investigating homicide. However, there are currently only limited methods available. Especially, once the PMI exceeds the duration of pre-adult development of the flies with the adult emergence, its determination is very approximate. Herein, we report the regular changes in hydrocarbon composition during the weathering process of the puparia in the field in Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae), one of the common species of necrophagous flies. Correlation analysis showed that the relative abundance of nearly all of the branched alkanes and alkenes decreased significantly with the weathering time. Especially, for 9 of the peaks, over 88% of the variance in their abundance was explained by weathering time. Further analysis indicated that the regular changes caused mainly by the different weathering rates of various hydrocarbons. Additionally, the weathering rates were found to depend on the chemical structure and molecular weight of the hydrocarbons. These results indicate strongly that hydrocarbon analysis is a powerful tool for determining the weathering time of the necrophagous fly puparia, and is expected to markedly improve the determination of the late PMI.
DOI: 10.1016/s1044-0305(97)00288-2
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