Microtubule-associated protein tau in human prostate cancer cells: isoforms, phosphorylation, and interactions.
Microtubule-associated protein tau in human prostate cancer cells: isoforms, phosphorylation, and interactions.
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DOI：
10.1002/jcb.22287
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发表时间：
2009-10-15
影响因子：
4
通讯作者：
Lee, Gloria
中科院分区：
文献类型：
--
作者：
Souter, Skye;Lee, Gloria
Tau is a microtubule-associated protein whose function has been investigated primarily in neurons. Recently, tau expression has been correlated with increased drug resistance in various cancers of non-neuronal tissues. In this report, we investigate the tau expressed in cancerous prostate lines ALVA-31, DU 145, and PC-3. Prostate cancer tau is heat-stable and highly phosphorylated, containing many of the modifications identified in Alzheimer’s Disease brain tau. RT-PCR and phosphatase treatment indicated that all six alternatively spliced adult brain tau isoforms are expressed in ALVA-31 cells and isoforms containing exon 6 as well as high molecular weight tau isoforms containing either exon 4A or a larger splice variant of exon 4A are also present. Consistent with its hyperphosphorylated state, a large proportion of ALVA-31 tau does not bind to microtubules, as detected by confocal microscopy and biochemical tests. Finally, endogenous ALVA-31 tau can interact with the p85 subunit of phosphatidylinositol 3-kinase, as demonstrated by co-immunoprecipitations and in vitro protein binding assays. Our results suggest that tau in prostate cancer cells does not resemble that from normal adult brain and support the hypothesis that tau is a multifunctional protein.
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DOI：
10.1083/jcb.101.4.1371
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Binder LI;Frankfurter A;Rebhun LI
通讯作者：
Rebhun LI
影响因子：
5.3
作者：
Lee, G;Thangavel, R;Ksiezak-Reding, H
通讯作者：
Ksiezak-Reding, H
影响因子：
2.5
作者：
Kohnken, R;Buerger, K;Hampel, H
通讯作者：
Hampel, H
影响因子：
12.7
作者：
MERCKEN, M;VANDERMEEREN, M;GHEUENS, J
通讯作者：
GHEUENS, J
胃癌中 tau 表达的减少可以确定紫杉醇治疗成功的候选者。
影响因子：
8.8
作者：
Mimori, K.;Sadanaga, N.;Yoshikawa, Y.;Ishikawa, K.;Hashimoto, M.;Tanaka, F.;Sasaki, A.;Inoue, H.;Sugimachi, K.;Mori, M.
通讯作者：
Mori, M.