Binding of human angiogenin inhibits actin polymerization.
Binding of human angiogenin inhibits actin polymerization.
复制标题
DOI：
10.1016/j.abb.2009.12.024
复制
发表时间：
2010-03-01
影响因子：
3.9
通讯作者：
Kostyukova AS
中科院分区：
文献类型：
--
作者：
Pyatibratov MG;Tolkatchev D;Plamondon J;Xu P;Ni F;Kostyukova AS
血管生成素是一种强有力的血管生成诱导剂，血管生成是血管形成的过程。它与内皮细胞和其他细胞相互作用，引起广泛的细胞反应，包括迁移、增殖和管状形成。血管生成素的一个重要靶点是血管内皮细胞-表面肌动蛋白，它们之间的相互作用可能是血管生成素诱导新生血管的重要步骤之一。基于血管生成素促进肌动蛋白聚合的早期迹象，我们在广泛的条件下研究了血管生成素与肌动蛋白之间的结合作用。我们发现，在亚生理浓度下，血管生成素并不促进聚合，而是通过隔离G-肌动蛋白来抑制聚合。在低KCl浓度下，血管生成素诱导形成无结构聚集体，如核磁共振所示，这可能是由于血管生成素形成低聚物的倾向所致。血管生成素与预制的F-肌动蛋白的结合不会引起肌动蛋白细丝的解聚，尽管它会导致肌动蛋白细丝变硬。原肌球蛋白和血管生成素与F-肌动蛋白的结合在足以使肌动蛋白细丝饱和的浓度下不具有竞争性。这些观察表明，血管生成素可能通过抑制G-肌动蛋白的聚合和改变F-肌动蛋白的物理性质而引起细胞骨架的变化。
Angiogenin is a potent inducer of angiogenesis, a process of blood vessel formation. It interacts with endothelial and other cells and elicits a wide range of cellular responses including migration, proliferation, and tube formation. One important target of angiogenin is endothelial cell-surface actin and their interaction might be one of essential steps in angiogenin-induced neovascularization. Based on earlier indications that angiogenin promotes actin polymerization, we studied the binding interactions between angiogenin and actin in a wide range of conditions. We showed that at subphysiological KCl concentrations, angiogenin does not promote, but instead inhibits polymerization by sequestering G-actin. At low KCl concentrations angiogenin induces formation of unstructured aggregates, which, as shown by NMR, may be caused by angiogenin's propensity to form oligomers. Binding of angiogenin to preformed F-actin does not cause depolymerization of actin filaments though it causes their stiffening. Binding of tropomyosin and angiogenin to F-actin is not competitive at concentrations sufficient for saturation of actin filaments. These observations suggest that angiogenin may cause changes in the cell cytoskeleton by inhibiting polymerization of G-actin and changing the physical properties of F-actin.
登录
查看更多内容
影响因子：
64.8
作者：
Mammoto, Akiko;Connor, Kip M.;Mammoto, Tadanori;Yung, Chong Wing;Huh, Dongeun;Aderman, Christopher M.;Mostoslavsky, Gustavo;Smith, Lois E. H.;Ingber, Donald E.
通讯作者：
Ingber, Donald E.
DOI：
10.1073/pnas.94.6.2204
发表时间：
1997-03-18
影响因子：
11.1
作者：
Hu, GF;Riordan, JF;Vallee, BL
通讯作者：
Vallee, BL
影响因子：
1.6
作者：
Holloway, DE;Hares, MC;Acharya, KR
通讯作者：
Acharya, KR
影响因子：
64.5
作者：
HITCHCOCK, SE;CARLSSON, L;LINDBERG, U
通讯作者：
LINDBERG, U
影响因子：
2.9
作者：
EDELHOCH, H
通讯作者：
EDELHOCH, H