Pathogen-origin horizontally transferred genes contribute to the evolution of Lepidopteran insects.
Pathogen-origin horizontally transferred genes contribute to the evolution of Lepidopteran insects.
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病原体起源水平转移基因有助于鳞翅目昆虫的进化
DOI：
10.1186/1471-2148-11-356
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发表时间：
2011-12-12
影响因子：
3.4
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Li ZW;Shen YH;Xiang ZH;Zhang Z
研究背景基因水平转移（Horizontal gene transfer，HGT）是遗传变异的来源之一，被普遍认为是促进寄主适应环境的重要因素。然而，令人信服的证据支持显着贡献的转移基因的进化后生动物recipiers.ResultsIn这项研究中，积累到目前为止的序列数据的基础上，我们使用了一个统一的方法，包括相似性搜索和系统发育分析，检测水平转移基因（HTGs）之间的原核生物和5种昆虫，包括果蝇，冈比亚按蚊，家蚕，赤拟谷盗和意大利蜜蜂。出乎意料的是，候选HTG在D.melanogaster、An.冈比亚和T. castaneum和Ap.根据其他信息，认为采用相同方法获得的意大利阿魏酸为污染物。结果表明，22种HTG中有14种仅在家蚕中检测到。此外，检测到的13种类型的家蚕HTG与其他鳞翅目超科物种的同源序列相同，表明这些HTG中的大多数来源于Ditrysia分支辐射之前的古老转移事件。根据系统发育拓扑图和BLAST检索结果，分别推断了这些基因的供体菌。至少有一半的预测供体生物可能是昆虫病原细菌。这些基因的生化功能包括糖基水解酶家族、氧化还原酶家族、氨基酸代谢和其他4类。这些基因可能有助于鳞翅目宿主在其古老谱系中的功能创新和适应性，与被子植物的多样性有关。重要的是，我们的研究结果表明，病原体可能是有利的生存和繁荣的主机通过有效的HGT事件在一个大的进化规模。
BackgroundHorizontal gene transfer (HGT), a source of genetic variation, is generally considered to facilitate hosts' adaptability to environments. However, convincing evidence supporting the significant contribution of the transferred genes to the evolution of metazoan recipients is rare.ResultsIn this study, based on sequence data accumulated to date, we used a unified method consisting of similarity search and phylogenetic analysis to detect horizontally transferred genes (HTGs) between prokaryotes and five insect species includingDrosophila melanogaster,Anopheles gambiae,Bombyx mori,Tribolium castaneumandApis mellifera. Unexpectedly, the candidate HTGs were not detected inD. melanogaster,An. gambiaeandT. castaneum, and 79 genes inAp. melliferasieved by the same method were considered as contamination based on other information. Consequently, 14 types of 22 HTGs were detected only in the silkworm. Additionally, 13 types of the detected silkworm HTGs share homologous sequences in species of other Lepidopteran superfamilies, suggesting that the majority of these HTGs were derived from ancient transfer events before the radiation of Ditrysia clade. On the basis of phylogenetic topologies and BLAST search results, donor bacteria of these genes were inferred, respectively. At least half of the predicted donor organisms may be entomopathogenic bacteria. The predicted biochemical functions of these genes include four categories: glycosyl hydrolase family, oxidoreductase family, amino acid metabolism, and others.ConclusionsThe products of HTGs detected in this study may take part in comprehensive physiological metabolism. These genes potentially contributed to functional innovation and adaptability of Lepidopteran hosts in their ancient lineages associated with the diversification of angiosperms. Importantly, our results imply that pathogens may be advantageous to the subsistence and prosperity of hosts through effective HGT events at a large evolutionary scale.
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