Development of circulating isolates of Plasmodium falciparum is accelerated in Anopheles vectors with reduced reproductive output.
Development of circulating isolates of Plasmodium falciparum is accelerated in Anopheles vectors with reduced reproductive output.
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DOI：
10.1371/journal.pntd.0011890
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发表时间：
2024-01
影响因子：
3.8
通讯作者：
中科院分区：
文献类型：
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冈比亚按蚊及其近亲种库鲁齐按蚊是疟疾原虫恶性疟原虫最有效的媒介。当这些物种的雌性以受感染的人类宿主为食时，卵子发生和寄生虫发育同时进行，但这些过程之间的相互作用尚不完全清楚。使用来自布基纳法索的多个天然恶性疟原虫分离株，我们表明在这两个载体中，通过破坏类固醇激素信号来扰乱卵子发生会导致卵囊加速生长，而且这种方式似乎取决于寄生虫和蚊子的基因。我们一致地发现，卵子数量与卵囊大小呈负相关，卵囊大小是衡量卵囊发育速度的一个指标。处于怀孕前状态的雌性卵囊生长速度也会大大加快，即在最初的血餐后不能发育卵子。总体而言，这些发现促进了我们对蚊子和寄生虫相互作用的理解，这些相互作用影响了疟疾流行地区恶性疟原虫的发展。疟疾是一种由疟原虫引起的传染病，每年继续影响数百万人。这些寄生虫是由按蚊在吸血过程中传播的，雌性蚊子需要吸血才能产卵。在被雌性蚊子摄取后，寄生虫开始在中肠发育，同时蚊子启动卵子发生，也就是卵的发育过程。在这里，我们研究了虫卵和寄生虫发育的伴随过程如何在野外来源的按蚊和疟原虫分离株中相互作用。我们发现，在这些自然发生的蚊子对中，卵子发生与寄生虫的发育呈负相关，因此产卵较少的蚊子藏匿着生长较快的卵囊。此外，当我们扰乱驱动卵子发生的信号通路时，卵囊的生长速度似乎取决于蚊子和寄生虫的基因。此外，当野外蚊子不能产卵时，它们的卵囊可能会生长得更快。这里揭示的关键蚊子和寄生虫发育过程之间的相互作用突显了疟原虫与蚊子生理的密切联系，并有助于开发既安全又有效的未来媒介控制工具。
Anopheles gambiae and its sibling species Anopheles coluzzii are the most efficient vectors of the malaria parasite Plasmodium falciparum. When females of these species feed on an infected human host, oogenesis and parasite development proceed concurrently, but interactions between these processes are not fully understood. Using multiple natural P. falciparum isolates from Burkina Faso, we show that in both vectors, impairing steroid hormone signaling to disrupt oogenesis leads to accelerated oocyst growth and in a manner that appears to depend on both parasite and mosquito genotype. Consistently, we find that egg numbers are negatively linked to oocyst size, a metric for the rate of oocyst development. Oocyst growth rates are also strongly accelerated in females that are in a pre-gravid state, i.e. that fail to develop eggs after an initial blood meal. Overall, these findings advance our understanding of mosquito-parasite interactions that influence P. falciparum development in malaria-endemic regions. Malaria, an infectious disease caused by Plasmodium parasites, continues to affect millions of people each year. These parasites are transmitted by Anopheles mosquitoes during blood feeding, which is required by females to produce eggs. After they are ingested by the female, parasites start developing in the midgut at the same time as the mosquito initiates oogenesis, the process of egg development. Here, we investigated how the concomitant processes of egg and parasite development interact in field-derived Anopheles and Plasmodium isolates. We found that in these naturally occurring pairs, oogenesis is negatively linked to parasite development, such that mosquitoes producing fewer eggs harbor faster growing oocysts. Furthermore, when we disrupt signaling pathways that drive oogenesis, oocyst growth is accelerated in a manner that appears to depend on both mosquito and parasite genotype. Additionally, when field mosquitoes fail to develop eggs, they are likely to have faster growing oocysts. The interaction between key mosquito and parasite developmental processes revealed here underscores how intimately linked Plasmodium are to mosquito physiology and can help inform the development of future vector control tools that are both safe and effective.
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影响因子：
2.1
作者：
Kaneko, O;Kimura, M;Tanabe, K
通讯作者：
Tanabe, K
影响因子：
3.7
作者：
Beck-Johnson LM;Nelson WA;Paaijmans KP;Read AF;Thomas MB;Bjørnstad ON
通讯作者：
Bjørnstad ON
影响因子：
3.2
作者：
Gimonneau, Geoffrey;Tchioffo, Majoline T.;Morlais, Isabelle
通讯作者：
Morlais, Isabelle
影响因子：
3.7
作者：
Harris, Caroline;Morlais, Isabelle;Cohuet, Anna
通讯作者：
Cohuet, Anna
影响因子：
5.7
作者：
CLEMENTS, AN;PATERSON, GD
通讯作者：
PATERSON, GD