Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice.
Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice.
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DOI：
10.1371/journal.pone.0128330
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Nakagata N
中科院分区：
文献类型：
--
作者：
Takeo T;Nakagata N
超数排卵是一种繁殖技术，通常用于生产基因工程小鼠。小鼠超数排卵包括给予马绒毛膜促性腺激素（eCG）以促进卵泡生长，然后给予人绒毛膜促性腺激素（hCG）以诱导排卵。以前，一些已发表的研究报告说，在ddY和野生来源的小鼠品系中，白藜芦醇抗血清（IAS）增加了排卵卵母细胞的数量。然而，IAS对C57 BL/6品系的影响尚未研究，C57 BL/6品系是用于生产基因工程小鼠的最广泛使用的近交系。此外，IAS和eCG（IASe）对超排卵治疗中排卵卵母细胞数量的联合作用尚未得到研究。本研究探讨了IAS和eCG对C57 BL/6系未成熟雌性小鼠超数排卵的影响。此外，我们评估了获得的卵母细胞的质量超排卵使用IASe体外受精（IVF）与精子从C57 BL/6或基因工程小鼠。通过胚胎移植检测新鲜胚胎和冷冻胚胎的发育能力。IAS或eCG给药对C57 BL/6雌性小鼠的排卵卵母细胞数量具有相似的影响。与单独施用IAS或eCG相比，施用IASe使排卵卵母细胞的数量增加至约3倍。来自使用IASe超数排卵的卵母细胞通常通过使用来自C57 BL/6小鼠的精子的IVF发育成2-细胞胚胎。通过IVF在C57 BL/6小鼠的卵母细胞和来自基因工程小鼠的精子之间产生的新鲜或冷冻保存的2-细胞胚胎在胚胎移植后通常发育成活的幼崽。总之，使用IASe进行超数排卵的新技术对于从基因工程小鼠产生大量卵母细胞和后代是非常有用的。
Superovulation is a reproductive technique generally used to produce genetically engineered mice. Superovulation in mice involves the administration of equine chorionic gonadotropin (eCG) to promote follicle growth and then that of human chorionic gonadotropin (hCG) to induce ovulation. Previously, some published studies reported that inhibin antiserum (IAS) increased the number of ovulated oocytes in ddY and wild-derived strains of mice. However, the effect of IAS on the C57BL/6 strain, which is the most widely used inbred strain for the production of genetically engineered mice, has not been investigated. In addition, the combined effect of IAS and eCG (IASe) on the number of ovulated oocytes in superovulation treatment has not been examined. In this study, we examined the effect of IAS and eCG on the number of ovulated oocytes in immature female mice of the C57BL/6 strain in superovulation treatment. Furthermore, we evaluated the quality of obtained oocytes produced by superovulation using IASe by in vitro fertilization (IVF) with sperm from C57BL/6 or genetically engineered mice. The developmental ability of fresh or cryopreserved embryos was examined by embryo transfer. The administration of IAS or eCG had a similar effect on the number of ovulated oocytes in C57BL/6 female mice. The number of ovulated oocytes increased to about 3-fold by the administration of IASe than by the administration of IAS or eCG alone. Oocytes derived from superovulation using IASe normally developed into 2-cell embryos by IVF using sperm from C57BL/6 mice. Fresh or cryopreserved 2-cell embryos produced by IVF between oocytes of C57BL/6 mice and sperm from genetically engineered mice normally developed into live pups following embryo transfer. In summary, a novel technique of superovulation using IASe is extremely useful for producing a great number of oocytes and offspring from genetically engineered mice.
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