Identification of a human T cell clone with the cytotoxic T lymphocyte and natural killer-like cytotoxic function against autologous mammary carcinoma and K562 line.
Identification of a human T cell clone with the cytotoxic T lymphocyte and natural killer-like cytotoxic function against autologous mammary carcinoma and K562 line.
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DOI：
10.1111/j.1349-7006.1989.tb01693.x
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发表时间：
1989-07
期刊：
影响因子：
--
通讯作者：
Kikuchi K
中科院分区：
文献类型：
--
作者：
Sato T;Okubo M;Wada Y;Sato N;Kikuchi K
一名60岁女性患者的胸膜渗出性淋巴细胞（PLED）对自体乳腺肿瘤细胞系HMC-2和NK敏感性K562细胞显示出较高的细胞毒性，尽管PLEL对几种同种异体肿瘤细胞系仅显示出较弱的细胞毒性潜力。我们成功地从PLEL批量群体中获得了7个细胞毒性T细胞克隆，并评估了这些淋巴细胞仅仅是自然杀伤（NK）样细胞或具有细胞毒性T淋巴细胞（CTL）和NK样细胞双重细胞毒性活性的可能性。这些被命名为TcHMC-2的克隆对HMC-2和K562细胞均显示出强细胞毒性。相比之下，同种异体人外周血来源的NK细胞不能杀死HMC-2靶标。此外，使用抗CD 3、CD 8和人MHC I类产物的单克隆抗体进行的TcHMC-2细胞毒性阻断研究表明，所有这些抗原分子均参与TcHMC. 2克隆对自体HMC-2细胞的细胞毒性，表明MHC I类抑制细胞毒性。这些数据表明，TcHMC-2克隆可能分别对自体HMC-2乳腺肿瘤和K562细胞具有CTL和NK样活性的双重细胞毒性。
Pleural exudative lymphocytes (PLED from a 60‐year‐old female patient showed high cytotoxicity against the autologous mammary tumor line, HMC‐2, and NK‐susceptible K562 cells, although PLEL demonstrated only weak cytotoxic potentials against several allogeneic tumor lines. We successfully obtained seven cytotoxic T cell clones from PLEL bulk populations, and assessed the possibility that these lymphocytes are simply natural killer (NK)‐like cells or have the dual cytotoxic activity of cytotoxic T lymphocytes (CTL) and NK‐like cells. These clones, designated as TcHMC‐2, showed strong cytotoxicity against both HMC‐2 and K562 cells. In contrast, allogeneic human peripheral blood‐derived NK cells could not kill HMC‐2 targets. Furthermore, a blocking study of TcHMC‐2 cytotoxicity using monoclonal antibodies against CD3, CD8 and human MHC class I products showed that all of these antigen molecules were involved in the cytotoxicity of TcHMC.2 clone against autologous HMC‐2 cells, indicating MHC class I recognitive cytotoxicity. These data indicate that the TcHMC‐2 clone may have dual cytotoxicity with CTL‐ and NK‐like activity against autologous HMC‐2 mammary tumor and K562 cells, respectively.
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影响因子：
56.9
作者：
MORGAN, DA;RUSCETTI, FW;GALLO, R
通讯作者：
GALLO, R
影响因子：
6.4
作者：
VOSE, BM;BONNARD, GD
通讯作者：
BONNARD, GD
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影响因子：
15.3
作者：
Yssel, H;Spits, H;de Vries, J E
通讯作者：
de Vries, J E
影响因子：
6.4
作者：
LIEBER, M;MAZZETTA, J;TODARO, G
通讯作者：
TODARO, G
影响因子：
64.8
作者：
HERCEND, T;REINHERZ, EL;RITZ, J
通讯作者：
RITZ, J