Silencing miR-150 Ameliorates Experimental Autoimmune Encephalomyelitis.
Silencing miR-150 Ameliorates Experimental Autoimmune Encephalomyelitis.
复制标题
沉默 miR-150 可改善实验性自身免疫性脑脊髓炎
DOI：
10.3389/fnins.2018.00465
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发表时间：
2018
影响因子：
4.3
通讯作者：
Dai R
中科院分区：
文献类型：
--
作者：
Hu Z;Cui Y;Qiao X;He X;Li F;Luo C;Wang S;Li C;Dai R
miR-150调节T细胞的成熟和分化，但它在多发性硬化症（MS）中的作用尚不清楚。在miR-150敲除（KO）小鼠中，我们检查了miR-150缺失对实验性自身免疫性脑脊髓炎（EAE）（MS的动物模型）疾病严重程度的影响。组织学染色和MBP免疫荧光染色显示miR-150缺失限制了脊髓中炎性脱髓鞘和轴突损伤的程度。流式细胞术显示，WT-EAE小鼠中CD 3+、CD 4+和CD 8 + T细胞增加，但miR-150缺失显著逆转了EAE介导的CD 3+、CD 4+和CD 8 + T细胞上调以及CD 19 + B细胞下调。此外，miR-150缺失降低了EAE诱导后脾脏和脊髓中IL-1β、IL-6、IL-17和TNF-α的mRNA表达。因此，miR-150缺失可能通过抑制中枢神经系统中激活的免疫应答和炎症来降低EAE的严重程度和脱髓鞘。
MiR-150 regulates maturation and differentiation of T cells but how it functions in multiple sclerosis (MS) is unclear. In miR-150 knockout (KO) mice, we examined the effect of miR-150 deletion on disease severity of experimental autoimmune encephalomyelitis (EAE), an animal model of MS. After deleting miR-150, EAE disease severity was reduced according to clinical score. Histological staining and MBP immunofluorescence staining revealed that miR-150 deletion limited the extent of inflammatory demyelination and axonal damage in the spinal cord. Flow cytometry showed that CD3+, CD4+, and CD8+ T cells were increased in WT-EAE mice, but miR-150 deletion significantly reversed EAE-mediated up-regulation of CD3+, CD4+, and CD8+ T cells and down-regulation of CD19+ B cells. In addition, miR-150 deletion reduced the mRNA expression of IL-1β, IL-6, IL-17, and TNF-α in spleen and spinal cord after EAE induction. Thus, miR-150 deletion reduces EAE severity and demyelination, probably through inhibiting the activated immune response and the inflammation in the central nervous system.
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影响因子：
12.3
作者：
Monticelli, Silvia;Ansel, K Mark;Xiao, Changchun;Socci, Nicholas D;Krichevsky, Anna M;Thai, To-Ha;Rajewsky, Nikolaus;Marks, Debora S;Sander, Chris;Rajewsky, Klaus;Rao, Anjana;Kosik, Kenneth S
通讯作者：
Kosik, Kenneth S
DOI：
10.1126/science.1139253
发表时间：
2007-04-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rodriguez A;Vigorito E;Clare S;Warren MV;Couttet P;Soond DR;van Dongen S;Grocock RJ;Das PP;Miska EA;Vetrie D;Okkenhaug K;Enright AJ;Dougan G;Turner M;Bradley A
通讯作者：
Bradley A
影响因子：
2.5
作者：
Martinelli-Boneschi, Filippo;Fenoglio, Chiara;Galimberti, Daniela
通讯作者：
Galimberti, Daniela
在主动脉交叉钳夹大鼠模型中，中度低温通过激活脊髓小胶质细胞增加迟发性麻痹的发生率
影响因子：
3.5
作者：
He, Liang;Xu, Jun-Mei;Dai, Ru-Ping
通讯作者：
Dai, Ru-Ping
影响因子：
3.5
作者：
Mann MK;Ray A;Basu S;Karp CL;Dittel BN
通讯作者：
Dittel BN