An integrated 4249 marker FISH/RH map of the canine genome.
An integrated 4249 marker FISH/RH map of the canine genome.
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DOI：
10.1186/1471-2164-5-65
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发表时间：
2004-09-13
期刊：
影响因子：
4.4
通讯作者：
Ostrander EA
中科院分区：
文献类型：
--
作者：
Breen M;Hitte C;Lorentzen TD;Thomas R;Cadieu E;Sabacan L;Scott A;Evanno G;Parker HG;Kirkness EF;Hudson R;Guyon R;Mahairas GG;Gelfenbeyn B;Fraser CM;André C;Galibert F;Ostrander EA
美国养犬俱乐部认可的156种狗提供了一个独特的机会来绘制基因变异的重要基因。每个品种都有一个明确的形态和行为特征，通常是由密切相关的个体故意杂交产生的，导致许多品种的遗传疾病率很高。了解狗的表型变异和疾病易感性的遗传基础，为剖析人类健康和生物学的遗传学提供了新的方法。为了便于遗传作图和克隆工作，我们构建了一个密集而准确的犬基因组图谱。所得到的资源包括4249个标记，并使用RHDF 5000 -2全基因组辐射杂交板构建。辐射杂交（RH）地图的特点是每900 Kb一个标记的密度，并包含1760个细菌人工染色体克隆（BAC）定位于1423个独特的位置，其中851个也已被绘制荧光原位杂交（FISH）。这两个数据集显示出极好的一致性。不包括Y染色体，该图谱的特征是每3.5 Mb有一个RH/FISH映射的BAC，平均每2 Mb有一个RH映射的BAC末端。对于2233个标记，已经建立了直链人类基因，允许鉴定狗和人类基因组之间的79个保守片段（CS），大大延长了大多数先前描述的CS的长度。这些结果提供了一个必要的资源，为犬基因组作图社区进行定位克隆实验，并提供了新的见解比较犬-人基因组图谱。
The 156 breeds of dog recognized by the American Kennel Club offer a unique opportunity to map genes important in genetic variation. Each breed features a defining constellation of morphological and behavioral traits, often generated by deliberate crossing of closely related individuals, leading to a high rate of genetic disease in many breeds. Understanding the genetic basis of both phenotypic variation and disease susceptibility in the dog provides new ways in which to dissect the genetics of human health and biology. To facilitate both genetic mapping and cloning efforts, we have constructed an integrated canine genome map that is both dense and accurate. The resulting resource encompasses 4249 markers, and was constructed using the RHDF5000-2 whole genome radiation hybrid panel. The radiation hybrid (RH) map features a density of one marker every 900 Kb and contains 1760 bacterial artificial chromosome clones (BACs) localized to 1423 unique positions, 851 of which have also been mapped by fluorescence in situ hybridization (FISH). The two data sets show excellent concordance. Excluding the Y chromosome, the map features an RH/FISH mapped BAC every 3.5 Mb and an RH mapped BAC-end, on average, every 2 Mb. For 2233 markers, the orthologous human genes have been established, allowing the identification of 79 conserved segments (CS) between the dog and human genomes, dramatically extending the length of most previously described CS. These results provide a necessary resource for the canine genome mapping community to undertake positional cloning experiments and provide new insights into the comparative canine-human genome maps.
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影响因子：
4.4
作者：
Priat, C;Hitte, C;Galibert, F
通讯作者：
Galibert, F
DOI：
10.1073/pnas.0831002100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Guyon, R;Lorentzen, TD;Ostrander, EA
通讯作者：
Ostrander, EA
影响因子：
64.5
作者：
Lin, L;Faraco, J;Mignot, E
通讯作者：
Mignot, E
影响因子：
56.9
作者：
Kirkness, EF;Bafna, V;Venter, JC
通讯作者：
Venter, JC
影响因子：
3.1
作者：
Hitte, C;Lorentzen, TD;Galibert, F
通讯作者：
Galibert, F