A longitudinal MRI study on lymph nodes histiocytosis of a xenograft cancer model.
A longitudinal MRI study on lymph nodes histiocytosis of a xenograft cancer model.
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DOI：
10.1371/journal.pone.0181043
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Reese T
中科院分区：
文献类型：
--
作者：
Jiménez-González M;Plaza-García S;Arizeta J;Bianchessi S;Trigueros C;Reese T
前哨淋巴结和原发性肿瘤恶性细胞的反应之间的关键过程和相互作用的检测和研究一直在努力。相反，一些常用的肿瘤动物模型，如人类癌症异种移植物，很少具有转移的特征。因此，很少评估淋巴结变化。我们认为，研究淋巴结反应可能有助于了解宿主对癌症的反应。在本研究中，我们使用不发生转移的胰腺肿瘤异种移植模型，探讨了与肿瘤生长平行的区域淋巴结改变的存在。我们通过在无胸腺裸鼠的左上腹皮下接种PANC-1（转移性人胰腺癌细胞系）建立了动物癌症模型。肿瘤动物，沿着对照组（n = 7 /组）进行磁共振成像（MRI），以在数周内跟踪肿瘤生长以及臂和腋淋巴结的变化。在研究结束时进行进一步的组织学分析。在第8周至第20周，肿瘤动物中不同淋巴结大小的个体平均值比对照动物大15-40%。肿瘤大小与淋巴结大小无相关性。淋巴结的组织学分析显示副皮质组织细胞增生症。组织学检查未发现淋巴结转移。在荷瘤动物中，组织细胞增多症与孤立的凋亡小体相关，通过人源性标志物的特异性免疫染色证实了人肿瘤细胞的迁移。在此建立的临床前模型中，没有转移以及淋巴结改变的病理学表现与窦组织细胞增生症患者的发现相似，后者与生存率提高相关。
Efforts are continuously made to detect and investigate the pivotal processes and interplay between the response of sentinel lymph node and malignant cells from a primary tumor. Conversely, some frequently used tumor animal models, such as human cancer xenografts, rarely feature metastasis. Therefore, lymph node alterations are seldom assessed. We consider that studying lymph node response could contribute to the understanding of host reaction to cancer. In the present study, we explored the presence of regional lymph node alterations in parallel with tumor growth using a pancreatic tumor xenograft model which does not develop metastasis. We established an animal cancer model by the subcutaneous inoculation of PANC-1 (a metastatic human pancreatic cancer cell line) in the left upper flank of athymic nude mice. Tumor animals, along with controls (n = 7 / group) were subjected to Magnetic Resonance Imaging (MRI) in order to follow tumor growth and brachial and axillary lymph nodes alterations over several weeks. Further histological analyses were performed at the end of the study. The individual average of the different lymph nodes sizes was 15–40% larger in the tumor animals compared to control animals at week 8 to week 20. The tumor size and lymph node size were not correlated. Histological analysis of the lymph nodes showed paracortical histiocytosis. No metastasis to lymph nodes could be detected by histology. In tumor bearing animals, histiocytosis was associated with isolated apoptotic bodies and migration of human tumoral cells was confirmed by specific immunostaining of human origin markers. The lack of metastasis as well as the pathological manifestation of the lymph node alteration in this pre-clinical model established here parallels findings in patients with sinus histiocytosis that is correlated with improved survival.
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影响因子：
3.7
作者：
Economopoulos V;Noad JC;Krishnamoorthy S;Rutt BK;Foster PJ
通讯作者：
Foster PJ
影响因子：
5.8
作者：
Ichii, S;Imai, Y;Irimura, T
通讯作者：
Irimura, T
影响因子：
3.5
作者：
Kikkawa, H;Imafuku, H;Oku, N
通讯作者：
Oku, N
影响因子：
51.1
作者：
Krag, David N.;Anderson, Stewart J.;Julian, Thomas B.;Brown, Ann M.;Harlow, Seth P.;Costantino, Joseph P.;Ashikaga, Takamaru;Weaver, Donald L.;Mamounas, Eleftherios P.;Jalovec, Lynne M.;Frazier, Thomas G.;Noyes, R. Dirk;Robidoux, Andre;Scarth, Hugh M. C.;Wolmark, Norman
通讯作者：
Wolmark, Norman
影响因子：
6.4
作者：
LIEBER, M;MAZZETTA, J;TODARO, G
通讯作者：
TODARO, G