Gentamicin-attenuated Leishmania infantum vaccine: protection of dogs against canine visceral leishmaniosis in endemic area of southeast of Iran.
Gentamicin-attenuated Leishmania infantum vaccine: protection of dogs against canine visceral leishmaniosis in endemic area of southeast of Iran.
复制标题
DOI：
10.1371/journal.pntd.0002757
复制
发表时间：
2014-04
影响因子：
3.8
通讯作者：
Phillips S
中科院分区：
文献类型：
--
作者：
Daneshvar H;Namazi MJ;Kamiabi H;Burchmore R;Cleaveland S;Phillips S
婴儿利什曼原虫（Leishmania infantum，L. infantum H-line）已经通过在庆大霉素压力下体外培养前鞭毛体而建立。使用来自利什曼病非流行区（55只接种疫苗和48只未接种疫苗）的103只未接种过疫苗的狗进行疫苗试验，这些狗被带到伊朗东南部的流行区。接种后即刻，在接种犬中未观察到局部和/或全身疾病指征。24个月后（4个白蛉传播季节），通过血清学、寄生虫学分析和临床检查评价疫苗的效力。免疫印迹法检测抗L.婴儿抗原，31只未接种疫苗的狗中有10只（32.2%）的血清，但在>100时血清阳性的接种疫苗的狗的血清中没有一只识别21 kDa的L.婴儿野生型（WT）。31只未接种疫苗的犬中有9只（29%）存在利什曼原虫DNA阳性，但接种疫苗的犬均为阴性。46只接种犬中有1只（2.2%）和31只未接种犬中有9只（29%）出现疾病临床体征。这些结果表明，庆大霉素减毒L。在流行区对犬内脏利什曼病有显著的保护作用。在伊朗东南部内脏利什曼病流行的地区，使用103只无利什曼病的狗进行了24个月的疫苗试验。用庆大霉素减毒婴儿利什曼原虫株免疫犬。接种婴儿利什曼原虫减毒品系后，在接种犬中未立即观察到局部和/或全身疾病指征。9/31（29%）未接种疫苗的犬，但没有接种疫苗的犬，通过PCR检测利什曼原虫DNA呈阳性。在蛋白质印迹中，31只未接种疫苗犬中有10只（32.2%）的血清识别婴儿利什曼原虫野生型的21 kDa抗原，但接种疫苗犬的血清均未识别。46只接种犬中有1只（2.2%）和31只未接种犬中有9只（29%）出现疾病临床体征。减毒的婴儿利什曼原虫在田间诱导了显著和强烈的抗婴儿利什曼原虫感染的保护作用。
An attenuated line of Leishmania infantum (L. infantum H-line) has been established by culturing promastigotes in vitro under gentamicin pressure. A vaccine trial was conducted using 103 naive dogs from a leishmaniosis non-endemic area (55 vaccinated and 48 unvaccinated) brought into an endemic area of southeast Iran. No local and/or general indications of disease were observed in the vaccinated dogs immediately after vaccination. The efficacy of the vaccine was evaluated after 24 months (4 sandfly transmission seasons) by serological, parasitological analyses and clinical examination. In western blot analysis of antibodies to L. infantum antigens, sera from 10 out of 31 (32.2%) unvaccinated dogs, but none of the sera from vaccinated dogs which were seropositive at >100, recognized the 21 kDa antigen of L. infantum wild-type (WT). Nine out of 31 (29%) unvaccinated dogs, but none of vaccinated dogs, were positive for the presence of Leishmania DNA. One out of 46 (2.2%) vaccinated dogs and 9 out of 31 (29%) unvaccinated dogs developed clinical signs of disease. These results suggest that gentamicin-attenuated L. infantum induced a significant and strong protective effect against canine visceral leishmaniosis in the endemic area. A 24 month vaccine trial was conducted using 103 leishmania free dogs in an area of southeast Iran endemic for visceral leishmaniosis. The dogs were vaccinated with gentamicin-attenuated line of Leishmania infantum. No local and/or general indications of disease were observed in the vaccinated dogs immediately after vaccination with an attenuated line of Leishmania infantum. Nine out of 31 (29%) unvaccinated dogs, but none of those vaccinated, were positive for presence of Leishmania DNA by PCR. In western blots, sera from 10 out of 31 (32.2%) unvaccinated dogs, but none of the sera from vaccinated dogs, recognized the 21 kDa antigen of Leishmania infantum wild-type. One out of 46 (2.2%) vaccinated dogs and 9 out of 31 (29%) unvaccinated dogs developed clinical signs of disease. The attenuated Leishmania infantum induced a significant and strong protective effect against Leishmania infantum infection in the field.
登录
查看更多内容
影响因子：
5.5
作者：
da Silva, VO;Borja-Cabrera, GP;de Sousa, CBP
通讯作者：
de Sousa, CBP
影响因子：
--
作者：
Otranto, D.;Paradies, P.;Capelli, G.
通讯作者：
Capelli, G.
影响因子：
3.3
作者：
Daneshvar, Hamid;Wyllie, Susan;Burchmore, Richard
通讯作者：
Burchmore, Richard
影响因子：
9.4
作者：
Mettler, M;Grimm, F;Deplazes, P
通讯作者：
Deplazes, P
影响因子：
2.1
作者：
Carrera, L;Fermin, ML;Alunda, JM
通讯作者：
Alunda, JM