The activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in the sera of patients with brain cancer.
The activity of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in the sera of patients with brain cancer.
复制标题
DOI：
10.1007/s11064-014-1402-3
复制
发表时间：
2014-12
影响因子：
4.4
通讯作者：
Szmitkowski, Maciej
中科院分区：
文献类型：
--
作者：
Jelski, Wojciech;Laniewska-Dunaj, Magdalena;Orywal, Karolina;Kochanowicz, Jan;Rutkowski, Robert;Szmitkowski, Maciej
人脑组织含有多种乙醇脱氢酶（ADH）同工酶，也具有醛脱氢酶（ALDH）活性。在我们最后的实验中，我们已经表明ADH和ALDH也存在于脑肿瘤细胞中。此外，总ADH和I类同工酶的活性显着高于癌组织比健康细胞。提示脑肿瘤患者血清中酶活性的变化可能反映了这些变化。本文对62例脑癌患者（胶质母细胞瘤36例，脑膜瘤26例）进行了血清常规生化检查。对于I类和II类ADH同工酶的活性和ALDH活性的测量，使用荧光法。用分光光度法测定ADH总活性和Ⅲ、Ⅳ类同工酶活性。在脑癌患者血清中发现I类乙醇脱氢酶同工酶的统计学显著增加。与对照组相比，患者组中该类同工酶的中位活性增加约24%。脑肿瘤患者的总乙醇脱氢酶活性也显著高于健康人（26%）。其它ADH同工酶和总ALDH活性无变化。脑肿瘤患者血清中总ADH和Ⅰ类乙醇脱氢酶同工酶活性的增高，可能是由于肿瘤细胞释放这种同工酶所致。
Human brain tissue contains various alcohol dehydrogenase (ADH) isoenzymes and possess also aldehyde dehydrogenase (ALDH) activity. In our last experiments we have shown that ADH and ALDH are present also in the brain tumour cells. Moreover the activities of total ADH and class I isoenzymes were significantly higher in cancer tissue than healthy cells. It can suggests that these changes may be reflected by enzyme activity in the serum of patients with brain cancer. Serum samples were taken for routine biochemical investigation from 62 patients suffering from brain cancer (36 glioblastoma, 26 meningioma). For the measurement of the activity of class I and II ADH isoenzymes and ALDH activity, the fluorometric methods were used. The total ADH activity and activity of class III and IV isoenzymes were measured by the photometric method. A statistically significant increase of class I alcohol dehydrogenase isoenzymes was found in the sera of patients with brain cancer. The median activity of this class isoenzyme in the patients group increased about 24 % in the comparison to the control level. The total alcohol dehydrogenase activity was also significantly higher (26 %) among patients with brain tumour than healthy ones. The activities of other tested ADH isoenzymes and total ALDH were unchanged. The increase of the activity of total ADH and class I alcohol dehydrogenase isoenzyme in the sera of patients with brain cancer seems to be caused by the release of this isoenzyme from tumour’s cells.
登录
查看更多内容
影响因子：
3.1
作者：
Jelski, W;Zalewski, B;Szmitkowski, M
通讯作者：
Szmitkowski, M
影响因子：
2.9
作者：
Mori, O;Haseba, T;Asano, G
通讯作者：
Asano, G
影响因子：
3.5
作者：
Estonius, M;Svensson, S;Hoog, JO
通讯作者：
Hoog, JO
影响因子：
3.1
作者：
Jelski, Wojciech;Kozlowski, Miroslaw;Szmitkowski, Maciej
通讯作者：
Szmitkowski, Maciej
影响因子：
3.5
作者：
KOIVUSALO, M;BAUMANN, M;UOTILA, L
通讯作者：
UOTILA, L