Toxicity of Flow Line, Durafill VS, and Dycal to dental pulp cells: effects of growth factors.
Toxicity of Flow Line, Durafill VS, and Dycal to dental pulp cells: effects of growth factors.
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DOI：
10.1016/j.joen.2010.03.013
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发表时间：
2010-07
影响因子：
4.2
通讯作者：
Lobner, Doug
中科院分区：
文献类型：
--
作者：
Furey, Alyssa;Hjelmhaug, Julie;Lobner, Doug
目的是确定生长因子治疗对牙髓细胞对两种复合修复材料（Flow Line和Durafill VS）和氢氧化钙盖髓材料（Dycal）毒性敏感性的影响。采用MTT法检测材料对体外培养的牙髓细胞的毒性。测试了六种不同生长因子影响毒性的能力。暴露于Flow Line或Durafill VS 24小时导致约40%的细胞死亡，而Dycal暴露导致约80%的细胞死亡。Flow Line和Durafill VS的毒性由氧化应激介导。检测的四种生长因子（BMP-2、BMP-7、EGF和TGF-β）降低了基础MTT值，同时使细胞对Flow Line和Durafill VS毒性具有抗性，但BMP-2除外，其使细胞对Flow Line更敏感。FGF-2处理未引起基础MTT代谢的变化，防止了Durafill VS的毒性，但增加了Flow Line的毒性。IGF-I处理增加了基础MTT代谢，并使细胞对Flow Line和Durafill VS毒性具有抗性。没有一种生长因子使细胞对Dycal毒性具有抗性。结果表明，生长因子可用于改变牙髓细胞对常用修复材料的敏感性。生长因子BMP-7、EGF、TGF-β和IGF-I提供了最佳效应特征，使细胞对Flow Line和Durafill VS毒性具有抗性。
The objective was to determine the effects of growth factor treatment on dental pulp cell sensitivity to toxicity of two composite restoration materials, Flow Line and Durafill VS, and a calcium hydroxide pulp capping material, Dycal. Toxicity of the dental materials to cultures of primary dental pulp cells was determined by the MTT metabolism assay. The ability of six different growth factors to influence the toxicity was tested. A 24 hour exposure to either Flow Line or Durafill VS caused approximately 40% cell death, while Dycal exposure caused approximately 80% cell death. The toxicity of Flow Line and Durafill VS was mediated by oxidative stress. Four of the growth factors tested (BMP-2, BMP-7, EGF, and TGF-β) decreased the basal MTT values while making the cells resistant to Flow Line and Durafill VS toxicity, except BMP-2 which made the cells more sensitive to Flow Line. Treatment with FGF-2 caused no change in basal MTT metabolism, prevented the toxicity of Durafill VS, but increased the toxicity of Flow Line. Treatment with IGF-I increased basal MTT metabolism and made the cells resistant to Flow Line and Durafill VS toxicity. None of the growth factors made the cells resistant to Dycal toxicity. The results indicate that growth factors can be used to alter the sensitivity of dental pulp cells to commonly used restoration materials. The growth factors BMP-7, EGF, TGF-β, and IGF-I provided the best profile of effects, making the cells resistant to both Flow Line and Durafill VS toxicity.
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影响因子：
3
作者：
Lobner, D
通讯作者：
Lobner, D
影响因子：
3
作者：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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影响因子：
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