Comparative evaluation of chitosan, cellulose acetate, and polyethersulfone nanofiber scaffolds for neural differentiation.
Comparative evaluation of chitosan, cellulose acetate, and polyethersulfone nanofiber scaffolds for neural differentiation.
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DOI：
10.1016/j.carbpol.2013.08.050
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发表时间：
2014-01
影响因子：
11.2
通讯作者：
Yarema, Kevin J.
中科院分区：
文献类型：
--
作者：
Du, Jian;Tan, Elaine;Hyo Jun Kim;Zhang, Allen;Bhattacharya, Rahul;Yarema, Kevin J.
关键词：
基于越来越多的证据表明生长表面的 3D 形貌和化学特征影响神经元分化，我们通过评估大鼠 PC12 系和人神经干细胞 (hNSC) 在壳聚糖 (CS)、醋酸纤维素 (CA) 和聚醚砜 (PES) 衍生的电纺纳米纤维上的细胞毒性、增殖和分化来结合这两个特征，这些纳米纤维具有相似的直径，以 200 至 500 nm 范围为中心。与 2D（例如平面）对照相比，纳米纤维材料均不具有细胞毒性；然而，纳米纤维支架上的增殖通常受到抑制，尽管与 PES 相比，多糖衍生材料的增殖程度较小。与 3D 基底上生长缓慢的趋势不同，在 CS 纳米纤维上分化的 hNSC 比 2D 对照增殖得更快，并且两种细胞类型在 CS 支架上都显示出增强的神经元分化迹象。总之，这些结果证明了当这种多糖用于电纺纳米纤维中定义的 3D 形貌时，CS 对于神经组织工程具有有益的属性。
Based on accumulating evidence that the 3D topography and the chemical features of a growth surface influence neuronal differentiation, we combined these two features by evaluating the cytotoxicity, proliferation, and differentiation of the rat PC12 line and human neural stem cells (hNSCs) on chitosan (CS), cellulose acetate (CA), and polyethersulfone (PES)-derived electrospun nanofibers that had similar diameters, centered in the 200 to 500 nm range. None of the nanofibrous materials were cytotoxic compared to 2D (e.g., flat surface) controls; however, proliferation generally was inhibited on the nanofibrous scaffolds although to a lesser extent on the polysaccharide-derived materials compared to PES. In an exception to the trend towards slower growth on the 3D substrates, hNSCs differentiated on the CS nanofibers proliferated faster than the 2D controls and both cell types showed enhanced indication of neuronal differentiation on the CS scaffolds. Together, these results demonstrate beneficial attributes of CS for neural tissue engineering when this polysaccharide is used in the context of the defined 3D topography found in electrospun nanofibers.
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