Effect of Endolymphatic Hydrops on Sound Transmission in Live Guinea Pigs Measured with a Laser Doppler Vibrometer.
Effect of Endolymphatic Hydrops on Sound Transmission in Live Guinea Pigs Measured with a Laser Doppler Vibrometer.
复制标题
用激光多普勒测振仪测量内淋巴积水对活豚鼠声音传输的影响。
DOI：
10.1155/2016/8648297
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发表时间：
2016
影响因子：
3.1
通讯作者：
Ren DD
中科院分区：
文献类型：
--
作者：
Ding CR;Xu XD;Wang XW;Jia XH;Cheng X;Liu X;Yang L;Tong BS;Chi FL;Ren DD
目标。本研究利用激光多普勒振动仪（LDV）研究了正常豚鼠和内淋巴水肿（EH）豚鼠低频听力损失的机制，以及耳膜、镫骨头和圆窗膜（RWM）的不同动态行为。方法。耳蜗切片采用苏木精和伊红（HE）染色，评价耳蜗的吸水率（HR）。观察听性脑干反应（ABR）和全载免疫染色。使用LDV评估脐、镫骨头和RWM对耳道声音的响应位移。结果。EH模型各回合平均HR值均大于对照组。EH组的ABR阈值显著高于对照组。根尖转HR与1000 Hz、次根尖转HR与2000 Hz ABR阈值升高呈显著正相关。耳蜗基底膜根尖、根尖下和基底上弯的FITC-phalloidin免疫染色显示第三排外毛细胞立体纤毛缺失和紊乱。EH患者耳部的拇趾、镫骨头和RWM位移普遍低于正常耳。在0.5 kHz时，eh诱导的镫骨头部和RWM运动差异显著。结论。LDV结果表明，EH时较高的内耳阻抗会影响耳蜗两个开口窗的动态行为，进而通过增加后负荷降低听骨链的振动，从而导致听觉功能障碍。振动的减少主要发生在低频，这与EH模型中根尖和亚根尖弯的形态变化有关。
Objective. This study aimed at describing the mechanism of hearing loss in low frequency and the different dynamic behavior of the umbo, the stapes head, and the round window membrane (RWM) between normal guinea pigs and those with endolymphatic hydrops (EH), using a laser Doppler vibrometer (LDV). Methods. Cochlear sections were stained with hematoxylin and eosin (HE) to evaluate the hydropic ratio (HR). Auditory brainstem responses (ABR) and whole-mount immunostaining were measured. Displacement of the umbo, stapes head, and RWM in response to ear-canal sound was evaluated using a LDV. Results. Mean HR values in EH model of all the turns are larger than the control group. The ABR threshold of the EH group was significantly higher than that of the control. Strong positive correlation was found between HR at apical turn and ABR threshold elevation at 1000 Hz and at subapical turn and ABR threshold elevation at 2000 Hz. FITC-phalloidin immunostaining of the cochlear basilar membrane in the apical, subapical, and suprabasal turns showed missing and derangement stereocilia of third-row outer hair cells. The umbo, stapes head, and RWM displacement in ears with EH was generally lower than that of normal ears. The EH-induced differences in stapes head and RWM motion were significant at 0.5 kHz. Conclusion. The LDV results suggested that the higher inner ear impedance in EH affected the dynamic behavior of the two opening windows of the cochlea and then reduced the vibration of the ossicular chain by increasing the afterload, resulting in acoustic dysfunction. The vibration reduction mainly occurred at low frequencies, which has related with the morphology changes of the apical and subapical turns in EH model.
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影响因子：
2.8
作者：
Gan, Rong Z.;Nakmali, Don;Zhang, Xiangming
通讯作者：
Zhang, Xiangming
影响因子：
2.8
作者：
Whittemore, KR;Merchant, SN;Rosowski, JJ
通讯作者：
Rosowski, JJ
影响因子：
1.4
作者：
Noguchi, Y;Nishida, H;Kitamura, K
通讯作者：
Kitamura, K
DOI：
10.1186/1916-0216-42-17
发表时间：
2013-02-25
期刊：
Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale
影响因子：
--
作者：
Beyea JA;Rohani SA;Ladak HM;Agrawal SK
通讯作者：
Agrawal SK
影响因子：
1.4
作者：
KLIS, SFL;BUIJS, J;SMOORENBURG, GF
通讯作者：
SMOORENBURG, GF