Laboratory demonstration of the vertical transmission of Rift Valley fever virus by Culex tarsalis mosquitoes.
Laboratory demonstration of the vertical transmission of Rift Valley fever virus by Culex tarsalis mosquitoes.
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实验室演示通过跗库蚊垂直传播裂谷热病毒。
DOI：
10.1371/journal.pntd.0009273
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发表时间：
2021-03
影响因子：
3.8
通讯作者：
Kading RC
中科院分区：
文献类型：
--
作者：
Bergren NA;Borland EM;Hartman DA;Kading RC
裂谷热病毒 (RVFV) 是一种通过蚊子传播的病毒，已被证明能够进入幼稚的地理区域。有限的现场证据表明，RVFV 从亲代蚊子垂直传播给后代，但迄今为止，这种机制尚未在实验室得到证实。此外，这种传播机制允许根据从卫星信息收集的降雨模式来预测裂谷热病毒流行病。然而，尽管垂直传播与动物流行事件的发生有关，但三十五年来，实验室确认垂直传播仍然是一个难以捉摸的研究目标。在此，我们提出了跗库蚊经口接触 RVFV 后垂直传播 RVFV 的初步证据。将来自三个连续性生殖周期的后代饲养至成虫，在每个发育阶段均证实具有感染性RVFV。吸食传染性血粉 7 天后，在亲代蚊子的卵巢组织中检测到病毒。早在第一个性营养周期就在子代中证实了感染，感染率范围为 2.0-10.0%。子代中的病毒滴度较低，这可能表明宿主机制抑制了复制。裂谷热病毒 (RVFV) 因其侵入原始地理区域的能力而构成重大威胁。此外，由于许多蚊子物种具有传播病毒的能力以及由于全球化的加剧而使病毒传播变得容易，裂谷热病毒代表了全球公共卫生风险。由于现场证据有限，RVFV 通过蚊子垂直传播早已被研究界所接受。然而，三十五年来，垂直传播的实验室确认仍然难以捉摸。我们首次提出了裂谷热病毒在北美易感病媒跗库蚊中垂直传播的实验室证据。我们提出的两项研究清楚地表明：1) RVFV 抗原在受感染蚊子的卵巢中积聚，2) 在感染性血粉后，RVFV 立即从父母传播给后代。
Rift Valley fever virus (RVFV) is a mosquito-transmitted virus with proven ability to emerge into naïve geographic areas. Limited field evidence suggests that RVFV is transmitted vertically from parent mosquito to offspring, but until now this mechanism has not been confirmed in the laboratory. Furthermore, this transmission mechanism has allowed for the prediction of RVFV epizootics based on rainfall patterns collected from satellite information. However, in spite of the relevance to the initiation of epizootic events, laboratory confirmation of vertical transmission has remained an elusive research aim for thirty-five years. Herein we present preliminary evidence of the vertical transmission of RVFV by Culex tarsalis mosquitoes after oral exposure to RVFV. Progeny from three successive gonotrophic cycles were reared to adults, with infectious RVFV confirmed in each developmental stage. Virus was detected in ovarian tissues of parental mosquitoes 7 days after imbibing an infectious bloodmeal. Infection was confirmed in progeny as early as the first gonotrophic cycle, with infection rates ranging from 2.0–10.0%. Virus titers among progeny were low, which may indicate a host mechanism suppressing replication. Rift Valley fever virus (RVFV) represents a significant threat in terms of its ability to emerge into naïve geographic areas. Furthermore, RVFV represents a global public health risk due to the ability of many mosquito species to transmit the virus and the ease with which the virus can be transported due to increased globalization. The vertical transmission of RVFV by mosquitoes has long been accepted by the research community due to limited field evidence. However, laboratory confirmation of vertical transmission has remained elusive for thirty-five years. We present the first laboratory evidence of vertical transmission of RVFV in the susceptible North American vector, Culex tarsalis. We present two studies that clearly show 1) the accumulation of RVFV antigen in the ovaries of infected mosquitoes and 2) the transmission of RVFV from parent to offspring immediately following an infectious blood meal.
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DOI：
10.3390/v10070362
发表时间：
2018-07-07
期刊：
Viruses
影响因子：
--
作者：
Hermance ME;Thangamani S
通讯作者：
Thangamani S
影响因子：
3.8
作者：
Golnar AJ;Turell MJ;LaBeaud AD;Kading RC;Hamer GL
通讯作者：
Hamer GL
影响因子：
4.4
作者：
Pepin M;Bouloy M;Bird BH;Kemp A;Paweska J
通讯作者：
Paweska J
DOI：
10.1073/pnas.0806490106
发表时间：
2009-01-20
影响因子：
11.1
作者：
Anyamba, Assaf;Chretien, Jean-Paul;Linthicum, Kenneth J.
通讯作者：
Linthicum, Kenneth J.
影响因子：
9.4
作者：
Garcia, S;Crance, JM;Garin, D
通讯作者：
Garin, D