Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling
Wheat bran feruloyl oligosaccharides ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling
复制标题
麦麸阿魏酰寡糖通过 Nrf2 信号传导改善 HepG2 细胞中 AAPH 诱导的氧化应激
DOI：
10.1016/j.jff.2016.06.012
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发表时间：
2016-08
影响因子：
5.6
通讯作者：
Sun, Baoguo
中科院分区：
文献类型：
--
作者：
Wang, Jing;Liu, Yingli;Gong, Lingxiao;Sun, Baoguo
研究了麦麸寡糖(FOS)对2，2‘-偶氮二(2-甲基丙酰胺)二盐酸盐(AAPH)诱导的HepG2细胞氧化应激的保护作用。通过细胞存活率、细胞内活性氧(ROS)生成和乳酸脱氢酶(LDH)漏出量来确定FOS对氧化攻击的细胞保护作用。通过测定超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)等II相解毒/抗氧化酶的活性和基因表达水平，研究果寡糖的抗氧化活性。FOS浓度与细胞存活率呈正相关，与LDH活性呈负相关。低聚果糖处理提高了超氧化物歧化酶、过氧化氢酶和GPX的活性和mRNA水平。此外，FOS组核因子E2相关因子(Nrf2)的蛋白表达与SOD、CAT和GPX蛋白水平呈正相关。结果表明，FOS可通过Nrf2信号转导途径减轻AAPH诱导的HepG2细胞氧化应激。
Protective effects of wheat bran feruloyl oligosaccharides (FOs) against 2, 2′-Azobis (2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells were investigated. The cytoprotective effects of FOs against oxidative challenge were determined by cell viability, intracellular reactive oxygen species (ROS) generation, and lactate dehydrogenase (LDH) leakage. The antioxidant activities of FOs were investigated by determining the activities and mRNA expression levels of phase II detoxifying/antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). FOs concentrations had a positive correlation with cell viability and a negative correlation with the LDH activity. FOs treatment increased the activities and mRNA levels of SOD, CAT, and GPx. Moreover, the nuclear factor E2 related factor (Nrf2) protein expression in the FOs group positively correlated with the SOD, CAT, and GPx protein levels. Thus the results demonstrated that FOs treatment could ameliorate AAPH-induced oxidative stress in HepG2 cells via Nrf2 signalling.
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影响因子：
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