Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice.
Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice.
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DOI：
10.3389/fmicb.2022.1034779
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发表时间：
2022
影响因子：
5.2
通讯作者：
Song, Xiao-Yan
中科院分区：
文献类型：
--
作者：
Zhang, Yu-Qiang;Zhang, Shan;Sun, Mei-Ling;Su, Hai-Nan;Li, Hao-Yang;Zhang, Yu-Zhong;Chen, Xiu-Lan;Cao, Hai-Yan;Song, Xiao-Yan
关键词：
由革兰氏阴性菌水稻黄单胞菌引起的白叶枯病水稻白叶枯病是水稻上危害最大的细菌性病害之一。由于化学杀菌剂的耐药性、毒性和环境问题，仍需要新的生物策略。木霉(Trichoderma spp.)虽然多肽能够抑制几种革兰氏阳性细菌和植物病原菌的生长，但目前尚不清楚多肽是否具有抑制水稻白叶枯病的活性。在本研究中，我们评价了长臂木霉SMF2产生的多肽Trichokonins A(TKA)对Xoo的抑制作用。体外抑菌活性分析表明，TKA对Xoo的生长有明显的抑制作用，最小抑菌浓度为54μg/mL，且TKA中的三种TKS均具有显著的抗Xoo活性。进一步的抑制机制分析表明，TKA处理导致了Xoo细胞形态的破坏和细胞内蛋白质、核酸等物质的释放，表明TKA破坏了Xoo细胞膜的通透性。致病力分析表明，27μg/mLTKA处理后，水稻叶片上的病斑长度显著减少82.2%。这项研究是关于多糖醇对革兰氏阴性杆菌的抗菌活性的第一次报道。因此，TKA在水稻白叶枯病防治中具有广阔的应用前景。
Bacterial leaf blight caused by Gram-negative pathogen Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive bacterial diseases on rice. Due to the resistance, toxicity and environmental issues of chemical bactericides, new biological strategies are still in need. Although peptaibols produced by Trichoderma spp. can inhibit the growth of several Gram-positive bacteria and plant fungal pathogens, it still remains unclear whether peptaibols have anti-Xoo activity to control bacterial leaf blight on rice. In this study, we evaluated the antibacterial effects of Trichokonins A (TKA), peptaibols produced by Trichoderma longibrachiatum SMF2, against Xoo. The in vitro antibacterial activity analysis showed that the growth of Xoo was significantly inhibited by TKA, with a minimum inhibitory concentration of 54 μg/mL and that the three TKs in TKA all had remarkable anti-Xoo activity. Further inhibitory mechanism analyses revealed that TKA treatments resulted in the damage of Xoo cell morphology and the release of intracellular substances, such as proteins and nucleic acids, from Xoo cells, suggesting the damage of the permeability of Xoo cell membrane by TKA. Pathogenicity analyses showed that the lesion length on rice leaf was significantly reduced by 82.2% when treated with 27 μg/mL TKA. This study represents the first report of the antibacterial activity of peptaibols against a Gram-negative bacterium. Thus, TKA can be of a promising agent in controlling bacterial leaf blight on rice.
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影响因子：
6.4
作者：
Fukuda S;Yamamoto R;Yanagisawa N;Takaya N;Sato Y;Riquelme M;Takeshita N
通讯作者：
Takeshita N
影响因子：
9.8
作者：
Laborte AG;Gutierrez MA;Balanza JG;Saito K;Zwart SJ;Boschetti M;Murty MVR;Villano L;Aunario JK;Reinke R;Koo J;Hijmans RJ;Nelson A
通讯作者：
Nelson A
影响因子：
5.2
作者：
Dos Santos IP;da Silva LC;da Silva MV;de Araújo JM;Cavalcanti Mda S;Lima VL
通讯作者：
Lima VL
抗菌 peptaibols 诱导烟草对烟草花叶病毒的防御反应和系统抗性
影响因子：
2.1
作者：
Luo, Yan;Zhang, Dan-Dan;Zhang, Yu-Zhong
通讯作者：
Zhang, Yu-Zhong
影响因子：
5.2
作者：
Marik, Tamas;Tyagi, Chetna;Kredics, Laszlo
通讯作者：
Kredics, Laszlo