Portable biosensor for monitoring cortisol in low-volume perspired human sweat.
Portable biosensor for monitoring cortisol in low-volume perspired human sweat.
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DOI：
10.1038/s41598-017-13684-7
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发表时间：
2017-10-17
影响因子：
4.6
通讯作者：
Prasad S
中科院分区：
文献类型：
--
作者：
Kinnamon D;Ghanta R;Lin KC;Muthukumar S;Prasad S
一个非法拉第无标记皮质醇生物传感器被证明使用二硫化钼纳米片集成到一个纳米多孔柔性电极系统。通过使用由限制半导电MoS 2纳米片的垂直对准金属电极组成的新型传感器堆叠设计来实现低体积（1-5 μL）感测。MoS 2纳米片表面功能化与皮质醇抗体对开发一种亲和生物传感器特定的生理相关范围的皮质醇（8.16至141.7 ng/mL）在出汗的人的汗水。通过使用电化学阻抗谱测量与皮质醇结合沿着的二硫化钼纳米片界面的阻抗变化来实现感测。该传感器的动态范围为1-500 ng/mL，检测限为1 ng/mL。使用人汗液中表达的代谢产物乙基葡糖苷酸进行专属性研究。进行了连续给药研究，在此期间，传感器能够在3小时以上的持续时间内区分四个皮质醇浓度范围（0.5、5、50、500 ng/mL）。该传感器的可转换性与便携式设备一起显示，证明了传感器的可比较的动态范围和检测限。当将测量值与台式仪器报告的阻抗值进行比较时，器械证明R2相关值为0.998。
A non-faradaic label-free cortisol biosensor was demonstrated using MoS2 nanosheets integrated into a nanoporous flexible electrode system. Low volume (1–5 μL) sensing was achieved through use of a novel sensor stack design comprised of vertically aligned metal electrodes confining semi-conductive MoS2 nanosheets. The MoS2 nanosheets were surface functionalized with cortisol antibodies towards developing an affinity biosensor specific to the physiological relevant range of cortisol (8.16 to 141.7 ng/mL) in perspired human sweat. Sensing was achieved by measuring impedance changes associated with cortisol binding along the MoS2 nanosheet interface using electrochemical impedance spectroscopy. The sensor demonstrated a dynamic range from 1–500 ng/mL with a limit of detection of 1 ng/mL. A specificity study was conducted using a metabolite expressed in human sweat, Ethyl Glucuronide. Continuous dosing studies were performed during which the sensor was able to discriminate between four cortisol concentration ranges (0.5, 5, 50, 500 ng/mL) for a 3+ hour duration. Translatability of the sensor was shown with a portable form factor device, demonstrating a comparable dynamic range and limit of detection for the sensor. The device demonstrated a R2 correlation value of 0.998 when comparing measurements to the reported impedance values of the benchtop instrumentation.
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影响因子：
4.6
作者：
Munje RD;Muthukumar S;Panneer Selvam A;Prasad S
通讯作者：
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影响因子：
4.2
作者：
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影响因子：
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作者：
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作者：
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通讯作者：
Sams, Clarence F.
影响因子：
5.8
作者：
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通讯作者：
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