Ethylene-insensitive mutants of Nicotiana tabacum exhibit drought stress resistance
Ethylene-insensitive mutants of Nicotiana tabacum exhibit drought stress resistance
复制标题
烟草乙烯不敏感突变体表现出干旱胁迫抗性
DOI：
10.1007/s10725-015-0116-0
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发表时间：
2016-05
影响因子：
4.2
通讯作者：
Chun-Hai Dong
中科院分区：
文献类型：
--
作者：
Honglin Wang;Feifei Wang;Fangfang Zheng;Lijuan Wang;Haixia Pei;Chun-Hai Dong
干旱是影响作物生长和产量的主要环境胁迫之一。气体激素乙烯被认为是植物生长和耐旱性的关键。基于暗生长的幼苗对乙烯的反应，在添加乙烯前体ACC（1-氨基环丙烷-1-羧酸）的培养基上进行烟草（Nicotiana tabacum）突变体的遗传筛选。在所分离的乙烯不敏感突变体中，通过甘露醇筛选获得了抗旱突变体。表型分析表明，这些突变体表现出增强的抗旱性，有较高的存活率，在严重水分亏缺的条件下，甘露醇渗透胁迫下更好的生长。突变体叶片失水速率降低，气孔开度减小。此外，过氧化物酶和超氧化物歧化酶的活性增加，可溶性总糖和脯氨酸的积累更高的突变体中检测到。烟草NtAP 2（Nicotiana tabacum中的APETALA 2，与拟南芥AtAP 2同源）和N. tabacum）基因在响应于空气干燥处理的植物中进行检测。本研究为植物抗旱突变体的筛选提供了一种可行的方法，同时也表明乙烯在植物耐旱性中起着重要作用。
Drought is one of the major environmental stresses that profoundly affect crop growth and yield. The gaseous hormone ethylene is believed to be critical for plant growth and drought tolerance. Based on the dark-grown seedling response to ethylene, a genetic screen for tobacco (Nicotiana tabacum) mutants on medium supplemented with ACC (1-aminocyclopropane-1-carboxylic acid), the precursor of ethylene, was performed. Among the ethylene insensitive mutants that were isolated, drought resistant mutants were subsequently obtained via screening on mannitol-containing medium. Phenotypic analyses revealed that these mutants exhibited enhanced drought resistance, having a higher percentage survival rate under conditions of severe water deficit, and better growth under mannitol osmotic stress. In the mutants, the leaf water loss rate was lowered and the leaf stomatal aperture was decreased. In addition, increases in peroxidase and superoxide dismutase activities and a higher accumulation of total soluble sugar and proline were detected in the mutant plants. The corresponding expression change of tobacco NtAP2 (APETALA2 in Nicotiana tabacum, homologous with AtAP2 from Arabidopsis thaliana) and NtERF (ethylene response factor in N. tabacum) genes was examined in the plants in response to air-drying treatments. Our study provides an applicable approach for the mutant screening of drought resistant plants, and the results suggest a crucial function of ethylene in plant drought tolerance.
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乙烯在脱水和再水合过程中玫瑰花瓣膨胀中的器官特异性作用
影响因子：
6.9
作者：
Liu D;Liu X;Meng Y;Sun C;Tang H;Jiang Y;Khan MA;Xue J;Ma N;Gao J
通讯作者：
Gao J
影响因子：
3.8
作者：
Yean Joo Seo;Jong-Beum Park;Yeon-Jeong Cho;Choonkyun Jung;H. Seo;Soon-Ki Park;B. Nahm;J. Song
通讯作者：
Yean Joo Seo;Jong-Beum Park;Yeon-Jeong Cho;Choonkyun Jung;H. Seo;Soon-Ki Park;B. Nahm;J. Song
影响因子：
7.4
作者：
Oñate-Sánchez, L;Singh, KB
通讯作者：
Singh, KB
DOI：
10.1016/j.plaphy.2005.01.015
发表时间：
2005-03
期刊：
Plant physiology and biochemistry : PPB
影响因子：
--
作者：
Mingjuan Tang;Shiyou Lü;Y. Jing;Xiangjuan Zhou;Jingwen Sun;S. Shen
通讯作者：
Mingjuan Tang;Shiyou Lü;Y. Jing;Xiangjuan Zhou;Jingwen Sun;S. Shen
影响因子：
56.9
作者：
BLEECKER, AB;ESTELLE, MA;KENDE, H
通讯作者：
KENDE, H