Electrospun fibers as a scaffolding platform for bone tissue repair.
Electrospun fibers as a scaffolding platform for bone tissue repair.
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DOI：
10.1002/jor.22367
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发表时间：
2013-09
影响因子：
2.8
通讯作者：
Zhang, Xinping
中科院分区：
文献类型：
--
作者：
Lyu, Seungyoun;Huang, Chunlan;Yang, Hong;Zhang, Xinping
本研究旨在探讨静电纺丝纤维直径和取向对骨髓基质细胞（BMSCs）分化和ECM组织化的影响，为制备用于骨缺损修复的骨膜模拟物提供理论依据。使用荧光显微镜、基因分析、电子扫描显微镜（SEM）和多光子激光扫描显微镜（MPLSM）在基于PLGA的随机和对齐纤维上分析细胞生长、分化和ECM组织。与相同大小的随机纤维相比，对齐纤维上的BMSC在第10天具有减少的ALP+集落数量。然而，在用成骨介质刺激后第21天，对齐纤维中的ALP+面积增加到与随机纤维相似的水平。与随机纤维相比，排列纤维上的BMSCs显示出更高的OSX和RUNX2表达。对脱细胞纺成纤维上的ECM的分析显示，根据纺成纤维的方向排列的高度组织化的ECM，具有在40 nm至2.4µm范围内的胶原纤维的宽尺寸分布。总之，我们的数据支持使用亚微米尺寸的静电纺丝纤维用于定向纤维组织模拟物的工程，如骨膜，用于引导骨修复和重建。
The purpose of the study is to investigate the effects of electrospun fiber diameter and orientation on differentiation and ECM organization of bone marrow stromal cells (BMSCs), in attempt to provide rationale for fabrication of a periosteum mimetic for bone defect repair. Cellular growth, differentiation, and ECM organization were analyzed on PLGA-based random and aligned fibers using fluorescent microscopy, gene analyses, electron scanning microscopy (SEM), and multiphoton laser scanning microscopy (MPLSM). BMSCs on aligned fibers had a reduced number of ALP+ colony at day 10 as compared to the random fibers of the same size. However, the ALP+ area in the aligned fibers increased to a similar level as the random fibers at day 21 following stimulation with osteogenic media. Compared with the random fibers, BMSCs on the aligned fibers showed a higher expression of OSX and RUNX2. Analyses of ECM on decellularized spun fibers showed highly organized ECM arranged according to the orientation of the spun fibers, with a broad size distribution of collagen fibers in a range of 40nm to 2.4µm. Taken together, our data support the use of submicron-sized electrospun fibers for engineering of oriented fibrous tissue mimetic, such as periosteum, for guided bone repair and reconstruction.
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