Identification of astrocytoma associated genes including cell surface markers.
Identification of astrocytoma associated genes including cell surface markers.
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DOI：
10.1186/1471-2407-4-39
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发表时间：
2004-07-21
期刊：
影响因子：
3.8
通讯作者：
Riggins GJ
中科院分区：
文献类型：
--
作者：
Boon K;Edwards JB;Eberhart CG;Riggins GJ
尽管付出了巨大的努力，但侵袭性星形细胞肿瘤的治疗选择仍然局限于手术和放疗，化疗对生存率的提高很少或没有提高。基因表达序列分析（SAGE）图谱的生成有望帮助鉴定星形细胞瘤相关基因和高表达的细胞表面基因作为分子治疗靶点。SAGE标签计数可以很容易地添加到公共表达数据库中，并迅速传播到全世界的研究工作中。我们生成并分析了25例原发性II级、III级和IV级星形细胞瘤的SAGE转录谱。这些图谱是作为癌症基因组解剖计划SAGE Genie的一部分生成的，并用于通过比较星形细胞瘤和正常脑转录来寻找候选治疗靶点的计算机搜索。采用Real-time PCR和免疫组织化学方法对2组独立的原发肿瘤中选择的候选靶基因进行验证。每个级别都鉴定出一组有限的肿瘤相关基因，其中包括以前与星形细胞瘤无关的基因（例如VCAM1， SMOC1和胸腺苷酸合成酶），细胞表面基因的比例很高。两个可用的抗体基因，水通道蛋白1和拓扑异构酶2A，显示了与转录水平预测一致的蛋白表达。这项在恶性和正常脑组织中转录的调查揭示了一小部分人类基因在恶性星形细胞瘤中被激活。除了提供通路生物学的见解外，我们还揭示和量化了细胞表面和细胞外星形细胞瘤基因的重要部分的表达。
Despite intense effort the treatment options for the invasive astrocytic tumors are still limited to surgery and radiation therapy, with chemotherapy showing little or no increase in survival. The generation of Serial Analysis of Gene Expression (SAGE) profiles is expected to aid in the identification of astrocytoma-associated genes and highly expressed cell surface genes as molecular therapeutic targets. SAGE tag counts can be easily added to public expression databases and quickly disseminated to research efforts worldwide. We generated and analyzed the SAGE transcription profiles of 25 primary grade II, III and IV astrocytomas. These profiles were produced as part of the Cancer Genome Anatomy Project's SAGE Genie , and were used in an in silico search for candidate therapeutic targets by comparing astrocytoma to normal brain transcription. Real-time PCR and immunohistochemistry were used for the validation of selected candidate target genes in 2 independent sets of primary tumors. A restricted set of tumor-associated genes was identified for each grade that included genes not previously associated with astrocytomas (e.g. VCAM1, SMOC1, and thymidylate synthetase), with a high percentage of cell surface genes. Two genes with available antibodies, Aquaporin 1 and Topoisomerase 2A, showed protein expression consistent with transcript level predictions. This survey of transcription in malignant and normal brain tissues reveals a small subset of human genes that are activated in malignant astrocytomas. In addition to providing insights into pathway biology, we have revealed and quantified expression for a significant portion of cell surface and extra-cellular astrocytoma genes.
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影响因子：
56.9
作者：
Saha, S;Bardelli, A;Vogelstein, B
通讯作者：
Vogelstein, B
影响因子：
1.2
作者：
Siu, I.-Mei;Lal, Anita;Riggins, Gregory J.
通讯作者：
Riggins, Gregory J.
影响因子：
82.9
作者：
Kononen, J;Bubendorf, L;Kallioniemi, OP
通讯作者：
Kallioniemi, OP
DOI：
10.1073/pnas.152324199
发表时间：
2002-08-20
影响因子：
11.1
作者：
Boon, K;Osório, EC;Riggins, GJ
通讯作者：
Riggins, GJ
影响因子：
11
作者：
Saadoun, S;Papadopoulos, MC;Bell, BA
通讯作者：
Bell, BA