Super-Resolution Ultrasound Localization Microscopy Using High-Frequency Ultrasound to Measure Ocular Perfusion Velocity in the Rat Eye.
Super-Resolution Ultrasound Localization Microscopy Using High-Frequency Ultrasound to Measure Ocular Perfusion Velocity in the Rat Eye.
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DOI：
10.3390/bioengineering10060689
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发表时间：
2023-06-06
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影响因子：
--
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眼部血管成像可以为眼部疾病的病理生理学提供新的见解。本研究提出了一种新型高频超分辨率超声定位显微镜（SRULM）技术，并评估了其测量眼内压（IOP）升高时大鼠眼体内灌注变化的能力。 VisualSonics Vevo F2 成像平台上的 38.4 MHz 中心频率线性阵列传感器用于收集全身微泡对比注射后大鼠眼后部的高帧频 (1 kHz) 射频数据。经过杂波和时空非局部均值过滤后，单个微泡被定位和跟踪。微泡轨迹累积超过 10,000 帧，以生成量化灌注速度和方向的血管图像。使用生理相关的受控流动状态进行算法验证，随后在大鼠眼睛上以 10 毫米汞柱 IOP 增量从 10 至 60 毫米汞柱进行体内实验。大鼠眼的后脉管系统，包括眼动脉、长睫状后动脉及其分支、视网膜中央动脉以及视网膜小动脉和小静脉均被成功可视化，并且在每个眼压水平上量化了速度。当眼压升高时，测量到动脉流量显着减少。高频 SRULM 可用于同时可视化和量化大鼠眼球后眼球和眼内血管系统的灌注速度。检测整个眼睛深度的眼灌注变化的能力可能有助于阐明缺血在青光眼等眼部疾病的病理生理学中的作用。
Imaging of the ocular vasculature can provide new insights into the pathophysiology of ocular diseases. This study proposes a novel high-frequency super-resolution ultrasound localization microscopy (SRULM) technique and evaluates its ability to measure in vivo perfusion changes in the rat eye at elevated intraocular pressure (IOP). A 38.4 MHz center frequency linear array transducer on a VisualSonics Vevo F2 imaging platform was used to collect high frame rate (1 kHz) radiofrequency data of the posterior rat eye following systemic microbubble contrast injection. Following clutter and spatiotemporal non-local means filtering, individual microbubbles were localized and tracked. The microbubble tracks were accumulated over 10,000 frames to generate vascular images quantifying perfusion velocity and direction. Experiments were performed using physiologic relevant controlled flow states for algorithm validation and subsequently performed in vivo on the rat eye at 10 mm Hg IOP increments from 10 to 60 mm Hg. The posterior vasculature of the rat eye, including the ophthalmic artery, long posterior ciliary arteries and their branches, central retinal artery and retinal arterioles and venules were successfully visualized, and velocities quantified at each IOP level. Significant reductions in arterial flow were measured as IOP was elevated. High-frequency SRULM can be used to visualize and quantify the perfusion velocity of the rat eye in both the retrobulbar and intraocular vasculature simultaneously. The ability to detect ocular perfusion changes throughout the depth of the eye may help elucidate the role ischemia has in the pathophysiology of ocular diseases such as glaucoma.
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影响因子：
4.1
作者：
Kim, Chung Young;Lee, Eun Ji;Kim, Tae-Woo
通讯作者：
Kim, Tae-Woo
DOI：
10.1111/j.1600-0420.2005.00567.x
发表时间：
2005-12-01
期刊：
ACTA OPHTHALMOLOGICA SCANDINAVICA
影响因子：
--
作者：
Martínez, A;Sánchez, M
通讯作者：
Sánchez, M
影响因子：
2.9
作者：
Christensen-Jeffries K;Couture O;Dayton PA;Eldar YC;Hynynen K;Kiessling F;O'Reilly M;Pinton GF;Schmitz G;Tang MX;Tanter M;van Sloun RJG
通讯作者：
van Sloun RJG
影响因子：
28.1
作者：
Heiles, Baptiste;Chavignon, Arthur;Couture, Olivier
通讯作者：
Couture, Olivier
影响因子：
3.5
作者：
Huang C;Zhang W;Gong P;Lok UW;Tang S;Yin T;Zhang X;Zhu L;Sang M;Song P;Zheng R;Chen S
通讯作者：
Chen S