Following the ontogeny of retinal waves: pan-retinal recordings of population dynamics in the neonatal mouse.
Following the ontogeny of retinal waves: pan-retinal recordings of population dynamics in the neonatal mouse.
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DOI：
10.1113/jphysiol.2013.262840
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发表时间：
2014-04-01
期刊：
影响因子：
--
通讯作者：
Sernagor E
中科院分区：
文献类型：
--
作者：
Maccione A;Hennig MH;Gandolfo M;Muthmann O;van Coppenhagen J;Eglen SJ;Berdondini L;Sernagor E
未成熟的视网膜在视觉体验开始之前的有限的发育期间产生扫过神经节细胞层的尖峰活动的自发波。波中编码的时空模式被认为对整个视觉系统的功能连接的布线具有指导意义。然而，由于传统记录技术的分辨率相对较低，视网膜波的个体发育仍然记录不多。在这里，我们使用大规模的，密集的，4096通道的多电极阵列，使我们能够记录从整个新生儿视网膜在近细胞的分辨率前所未有的细节，从出生到眼睛张开的小鼠视网膜波的时空特征。我们发现，早期胆碱能波传播随机轨迹大面积低神经节细胞招聘。当GABAA信号成熟时，它们变得更慢，更小和更密集，如出生后第7天（P）之后发生的那样。谷氨酸能的影响占主导地位，从P10，符合活动动力学的深刻变化。此时，波不再是随机的，并且开始显示出局限于少数局部热点的重复轨迹。这些热点随着时间的推移逐渐平铺视网膜，并在眼睛睁开后消失。我们的观察表明，视网膜波经历重大的时空变化在个体发育。我们的研究结果支持的假设，胆碱能波引导视网膜目标的细化，以及多巴胺能波也可能支持布线的视网膜感受野。
The immature retina generates spontaneous waves of spiking activity that sweep across the ganglion cell layer during a limited period of development before the onset of visual experience. The spatiotemporal patterns encoded in the waves are believed to be instructive for the wiring of functional connections throughout the visual system. However, the ontogeny of retinal waves is still poorly documented as a result of the relatively low resolution of conventional recording techniques. Here, we characterize the spatiotemporal features of mouse retinal waves from birth until eye opening in unprecedented detail using a large-scale, dense, 4096-channel multielectrode array that allowed us to record from the entire neonatal retina at near cellular resolution. We found that early cholinergic waves propagate with random trajectories over large areas with low ganglion cell recruitment. They become slower, smaller and denser when GABAA signalling matures, as occurs beyond postnatal day (P) 7. Glutamatergic influences dominate from P10, coinciding with profound changes in activity dynamics. At this time, waves cease to be random and begin to show repetitive trajectories confined to a few localized hotspots. These hotspots gradually tile the retina with time, and disappear after eye opening. Our observations demonstrate that retinal waves undergo major spatiotemporal changes during ontogeny. Our results support the hypotheses that cholinergic waves guide the refinement of retinal targets and that glutamatergic waves may also support the wiring of retinal receptive fields.
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DOI：
10.1523/jneurosci.3112-11.2011
发表时间：
2011-08-24
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Hennig MH;Grady J;van Coppenhagen J;Sernagor E
通讯作者：
Sernagor E
影响因子：
16.2
作者：
Akrouh A;Kerschensteiner D
通讯作者：
Kerschensteiner D
影响因子：
6.1
作者：
Berdondini, Luca;Imfeld, Kilian;Martinoia, Sergio
通讯作者：
Martinoia, Sergio
影响因子：
64.5
作者：
Furukawa, T;Morrow, EM;Cepko, CL
通讯作者：
Cepko, CL
影响因子：
16.2
作者：
Feller, MB;Butts, DA;Shatz, CJ
通讯作者：
Shatz, CJ