The microbiota of the surface, dermis and subcutaneous tissue of dog skin.
The microbiota of the surface, dermis and subcutaneous tissue of dog skin.
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DOI：
10.1186/s42523-020-00050-8
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发表时间：
2020-09-22
影响因子：
4.7
通讯作者：
Cuscó A
中科院分区：
文献类型：
--
作者：
García-Fonticoba R;Ferrer L;Francino O;Cuscó A
使用高度敏感的分子技术的研究已经检测到人类表皮下的细菌群落。根据它们的丰度和组成，这一发现可能具有临床相关性。本研究的目的是确定是否可以使用两种不同的方法在没有皮肤疾病的狗的真皮和皮下组织中检测到细菌：传统培养和使用下一代测序 (NGS) 对细菌 16S rRNA 基因 V4 区域进行 DNA 测序。该研究纳入了七只健康的狗，并从每个受试者身上收集了两组样本。样本组由6毫米腹部皮肤活检样本（包括表皮、真皮和皮下组织）、皮肤表面拭子和用于污染控制的环境空白样本组成。每只狗的一组样本被提交用于细菌培养，另一组样本用于细菌 DNA 扩增和测序。通过需氧微生物培养方法，在 7 个皮肤表面拭子样品中的 5 个样品中分离出 5 个不同的细菌属（葡萄球菌、芽孢杆菌、棒状杆菌、链球菌和肠球菌），而其他两个样品没有生长。虽然可以从表皮、真皮和皮下组织样本中扩增出一些 DNA，但 NGS 的结果与空白的结果相似。当用需氧微生物培养方法进行研究时，狗的真皮和皮下组织是无菌的。 NGS 技术可检测到一些细菌 DNA，类似于空白中检测到的信号，这不支持真皮或皮下组织中存在微生物群。
Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. The aim of this study was to determine if bacteria can be detected in the dermis and subcutaneous tissue of dogs without cutaneous disease using two different approaches: traditional cultures and DNA sequencing of the V4 region of bacterial 16S rRNA gene using next-generation sequencing (NGS). Seven healthy dogs were included in the study, and two sets of samples were collected from each subject. Sample sets were composed of a 6-mm abdominal skin biopsy, including epidermis, dermis, and subcutis, a skin surface swab, and an environmental blank sample for contamination control. One set of samples from each dog was submitted for bacterial culture and the other one for bacterial DNA amplification and sequencing. Five different bacterial genera (Staphylococcus, Bacillus, Corynebacterium, Streptococcus, and Enterococcus) were isolated in five out of the seven skin surface swab samples with aerobic microbiological culture methods, while no growth was obtained from the other two samples. Although some DNA could be amplified from epidermal, dermal, and subcutaneous tissue samples, the results of the NGS were similar to those of the blanks. When investigated with aerobic microbiological culture methods, the dermis and subcutaneous tissue of dogs are sterile. NGS techniques lead to the detection of some bacterial DNA, similar to the signal detected in blanks, which does not support the presence of a microbiota in dermis or subcutaneous tissue.
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影响因子：
3.7
作者：
Abayasekara LM;Perera J;Chandrasekharan V;Gnanam VS;Udunuwara NA;Liyanage DS;Bulathsinhala NE;Adikary S;Aluthmuhandiram JVS;Thanaseelan CS;Tharmakulasingam DP;Karunakaran T;Ilango J
通讯作者：
Ilango J
影响因子：
15.5
作者：
Cuscó A;Belanger JM;Gershony L;Islas-Trejo A;Levy K;Medrano JF;Sánchez A;Oberbauer AM;Francino O
通讯作者：
Francino O
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1038/jid.2011.417
发表时间：
2012-03
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Kong HH;Segre JA
通讯作者：
Segre JA
影响因子：
16.6
作者：
通讯作者：
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