Real-Time Monitoring of Insulin Using a Graphene Field-Effect Transistor Aptameric Nanosensor.
Real-Time Monitoring of Insulin Using a Graphene Field-Effect Transistor Aptameric Nanosensor.
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DOI：
10.1021/acsami.7b07684
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发表时间：
2017-08-23
影响因子：
9.5
通讯作者：
Lin Q
中科院分区：
文献类型：
--
作者：
Hao Z;Zhu Y;Wang X;Rotti PG;DiMarco C;Tyler SR;Zhao X;Engelhardt JF;Hone J;Lin Q
本文提出了一种方法，实时，无标记，具体的，和灵敏的监测胰岛素使用石墨烯适体纳米传感器。纳米传感器被配置为场效应晶体管，其石墨烯基导电通道用富含鸟嘌呤的IGA 3适体官能化。带负电荷的适体在与胰岛素结合时折叠成紧凑且稳定的反平行或平行G-四链体构象，导致石墨烯的载流子密度变化，并因此导致电导变化。然后测量电导率的变化，以实现对胰岛素水平的实时监测。测试表明，纳米传感器提供了一个估计的检测限低至35 pM，并在Krebs-Ringer碳酸氢盐缓冲液，一个标准的胰岛灌注介质的功能。这些结果表明，这种方法在无标记监测胰岛素和及时预测准确的胰岛素剂量在临床诊断中的潜在效用。
This paper presents an approach to the real-time, label-free, specific, and sensitive monitoring of insulin using a graphene aptameric nanosensor. The nanosensor is configured as a field-effect transistor, whose graphene-based conducting channel is functionalized with a guanine-rich IGA3 aptamer. The negatively charged aptamer folds into a compact and stable antiparallel or parallel G-quadruplex conformation upon binding with insulin, resulting in a change in the carrier density, and hence the electrical conductance, of the graphene. The change in the electrical conductance is then measured to enable the real-time monitoring of insulin levels. Testing has shown that the nanosensor offers an estimated limit of detection down to 35 pM and is functional in Krebs–Ringer bicarbonate buffer, a standard pancreatic islet perfusion medium. These results demonstrate the potential utility of this approach in label-free monitoring of insulin and in timely prediction of accurate insulin dosage in clinical diagnostics.
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影响因子：
12.6
作者：
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通讯作者：
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影响因子：
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作者：
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