Tubular perfusion system culture of human mesenchymal stem cells on poly-L-lactic acid scaffolds produced using a supercritical carbon dioxide-assisted process.
Tubular perfusion system culture of human mesenchymal stem cells on poly-L-lactic acid scaffolds produced using a supercritical carbon dioxide-assisted process.
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DOI：
10.1002/jbm.a.34191
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发表时间：
2012-10
影响因子：
4.9
通讯作者：
Reverchon, Ernesto
中科院分区：
文献类型：
--
作者：
Pisanti, Paola;Yeatts, Andrew B.;Cardea, Stefano;Fisher, John P.;Reverchon, Ernesto
体外人间充质干细胞 (hMSC) 增殖和分化取决于支架设计参数和特定培养条件。在这项研究中，我们研究了支架微观结构如何影响灌注生物反应器系统中的 hMSC 行为。聚-l-乳酸 (PLLA) 支架是使用超临界二氧化碳 (SC-CO2) 凝胶干燥制备的。这种生产方法产生了具有纳米结构的支架。为了引入微孔结构，除了该技术之外，还使用致孔剂浸出来生产平均孔径为 100、250 和 500 µm 的支架。然后将这些支架在孔板中静态培养或在管式灌注系统（TPS）生物反应器中动态培养。结果表明，hMSC 能够在所有支架上附着并保持活力，在生物反应器培养支架的 250 µm 和 500 µm 孔径以及静态培养支架的 100 µm 孔径中具有更高的增殖能力。与静态培养相比，TPS 培养中的成骨细胞分化增强，在 250 µm 孔径组中观察到碱性磷酸酶表达最高。还分析了骨形态发生蛋白-2，在 250 µm 和 500 µm 孔径生物反应器培养样品中表达水平最高。这些结果证明了细胞对孔径的反应以及动态培养增强这些效应的能力。
In vitro human mesenchymal stem cell (hMSC) proliferation and differentiation is dependent on scaffold design parameters and specific culture conditions. In this study, we investigate how scaffold microstructure influences hMSC behavior in a perfusion bioreactor system. Poly-l-lactic acid (PLLA) scaffolds are fabricated using supercritical carbon dioxide (SC-CO2) gel drying. This production method results in scaffolds fabricated with nanostructure. To introduce a microporous structure, porogen leaching was used in addition to this technique to produce scaffolds of average pore size of 100, 250, and 500 µm. These scaffolds were then cultured in static culture in well plates or dynamic culture in the tubular perfusion system (TPS) bioreactor. Results indicated that hMSCs were able to attach and maintain viability on all scaffolds with higher proliferation in the 250 µm and 500 µm pore sizes of bioreactor cultured scaffolds and 100 µm pore size of statically cultured scaffolds. Osteoblastic differentiation was enhanced in TPS culture as compared to static culture with the highest alkaline phosphatase expression observed in the 250 µm pore size group. Bone morphogenetic protein-2 was also analyzed and expression levels were highest in the 250 µm and 500 µm pore size bioreactor cultured samples. These results demonstrate cellular response to pore size as well as the ability of dynamic culture to enhance these effects.
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影响因子：
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作者：
Kim, Kyobum;Dean, David;Lu, Anqi;Mikos, Antonios G.;Fisher, John P.
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影响因子：
3.1
作者：
He, Liumin;Liu, Bin;Ramakrishna, S.
通讯作者：
Ramakrishna, S.
影响因子：
3.9
作者：
Cardea, S.;Pisanti, P.;Reverchon, E.
通讯作者：
Reverchon, E.
影响因子：
14
作者：
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通讯作者：
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影响因子：
4.2
作者：
Reverchon, Ernesto;Pisanti, Paola;Cardea, Stefano
通讯作者：
Cardea, Stefano