3D-printed nanocomposite scaffolds with tunable magnesium ionic microenvironment induce in situ bone tissue regeneration
3D-printed nanocomposite scaffolds with tunable magnesium ionic microenvironment induce in situ bone tissue regeneration
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具有可调镁离子微环境的3D打印纳米复合材料支架可诱导原位骨组织再生
DOI：
10.1016/j.apmt.2019.07.012
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发表时间：
2019-09
影响因子：
8.3
通讯作者：
Kelvin W.K. Yeung
中科院分区：
文献类型：
--
作者：
Jie Shen;Wenhao Wang;Xinyun Zhai;Bo Chen;Wei Qiao;Wan Li;Penghui Li;Ying Zhao;Yuan Meng;Shi Qian;Xuanyong Liu;Paul K. Chu;Kelvin W.K. Yeung
局部组织微环境能够调节细胞与细胞的相互作用，从而导致有效的组织修复。本研究旨在证明骨组织中可调的镁离子（Mg 2+）微环境可以显著诱导骨缺损修复。该概念可以通过使用新制造的纳米复合材料来实现，该纳米复合材料包括定制的共聚物聚己内酯-共-聚（乙二醇）-共-聚己内酯（PCL-PEG-PCL）和表面改性的氧化镁（MgO）纳米颗粒。在这项研究中，增材制造（AM）技术已被采用，以帮助设计多孔三维（3D）支架与可调Mg 2+微环境。结果表明，新型共聚物的润湿性和印刷适性较PCL聚合物有较大的改善。此外，当MgO纳米颗粒掺入到新合成的亲水性共聚物基体中时，它可以导致显著增加的压缩模量。在体外研究中，与纯PCL和PCL-PEG-PCL共聚物对照相比，所制备的具有低浓度Mg 2+微环境的纳米复合支架不仅表现出更好的细胞相容性，而且在体外显著增强了成骨分化。在动物研究中，我们还发现在相同的3D打印支架中可以观察到上级和早期的骨形成和组织矿化。然而，高浓度的Mg 2+纳米复合支架由于骨组织微环境中过量的镁离子而危及原位骨组织再生能力。最后，本研究表明，在骨组织微环境中具有受控镁浓度的纳米复合3D支架可以有效地促进骨缺损修复。
Local tissue microenvironment is able to regulate cell-to-cell interaction that leads to effective tissue repair. This study aims to demonstrate a tunable magnesium ionic (Mg2+) microenvironment in bony tissue that can significantly induce bone defect repair. The concept can be realized by using a newly fabricated nanocomposite comprising of custom-made copolymer polycaprolactone-co-poly(ethylene glycol)-co-polycaprolactone (PCL-PEG-PCL) and surface-modified magnesium oxide (MgO) nanoparticles. In this study, additive manufacturing (AM) technology had been adopted to help design the porous three-dimensional (3D) scaffolds with tunable Mg2+microenvironment. We found that the wettability and printability of new copolymer had been improved as compared with that of PCL polymer. Additionally, when MgO nanoparticles incorporated into the newly synthesized hydrophilic copolymer matrix, it could lead to increased compressive moduli significantly. In thein vitrostudies, the fabricated nanocomposite scaffold with low concentration of Mg2+microenvironment not only demonstrated better cytocompatibility, but also remarkably enhanced osteogenic differentiationin vitroas compared with the pure PCL and PCL-PEG-PCL co-polymer controls. In the animal studies, we also found that superior and early bone formation and tissue mineralization could be observed in the same 3D printed scaffold. However, the nanocomposite scaffold with high concentration of Mg2+jeopardized thein situbony tissue regeneration capability due to excessive magnesium ions in bone tissue microenvironment. Lastly, this study demonstrates that the nanocomposite 3D scaffold with controlled magnesium concentration in bone tissue microenvironment can effectively promote bone defect repair.
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影响因子：
3.2
作者：
Leidi, Marzia;Dellera, Federica;Maier, Jeanette A. M.
通讯作者：
Maier, Jeanette A. M.
双膦酸盐-镁 (Mg2) 配位稳定的自组装可注射纳米复合水凝胶通过双交联调节封装干细胞的分化
影响因子：
19
作者：
Zhang Kunyu;Feng Qian;Xu Jianbin;Xu Xiayi;Tian Feng;Yeung Kelvin W K;Bian Liming
通讯作者：
Bian Liming
影响因子：
3.5
作者：
Holmes B;Bulusu K;Plesniak M;Zhang LG
通讯作者：
Zhang LG
影响因子：
29
作者：
Jeffery E;Wing A;Holtrup B;Sebo Z;Kaplan JL;Saavedra-Peña R;Church CD;Colman L;Berry R;Rodeheffer MS
通讯作者：
Rodeheffer MS
DOI：
10.1002/jbm.b.31788
发表时间：
2011-04-01
影响因子：
3.4
作者：
Fu, Shao Zhi;Wang, Xiu Hong;Qian, Zhi Yong
通讯作者：
Qian, Zhi Yong