Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption.
Artificial selection for odor-guided behavior in Drosophila reveals changes in food consumption.
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DOI：
10.1186/s12864-017-4233-1
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发表时间：
2017-11-13
期刊：
影响因子：
4.4
通讯作者：
Rollmann SM
中科院分区：
文献类型：
--
作者：
Brown EB;Patterson C;Pancoast R;Rollmann SM
嗅觉系统使生物体能够检测环境中的化学线索，并可以发出食物的可用性或捕食者的存在的信号。因此，对这些化学信号的适当行为反应对于生物体的生存非常重要，并且可以影响生物体的寿命和食物消耗等特征。然而，了解气味引导行为背后的遗传机制、其他性状的相关反应，以及这些如何限制或促进其进化，仍然是一个重要的挑战。在这里，我们对四种化合物、两种芳香族化合物（4-乙基愈创木酚和4-甲基苯酚）和两种酯（己酸甲酯和乙酸乙酯）进行了人工选择，进行了三十代人的吸引和厌恶行为反应。对气味引导行为的人工选择揭示了对四种化合物中每一种的选择的对称反应。然后，我们研究了气味引导行为的选择是否会导致生活史特征和/或食物消耗的相关反应。我们发现，选择对芳香剂的行为反应后，食物消耗会发生变化。在许多情况下，为增加对芳香剂的吸引力而选择的品系显示出食品消耗的增加。然后，我们对针对 4-乙基愈创木酚反应而选择的品系进行了 RNA 测序，以确定与气味引导行为及其对食物消耗的影响相关的候选基因。我们鉴定了 91 个在品系之间差异表达的基因，其中许多与代谢过程相关。 RNAi 介导的选定候选基因的敲低进一步支持了它们在气味引导行为和/或食物消耗中的作用。这项研究确定了气味引导行为变化背后的新基因，并进一步阐明了嗅觉和进食之间相互关系的遗传机制。本文的在线版本 (10.1186/s12864-017-4233-1) 包含补充材料，可供授权用户使用。
The olfactory system enables organisms to detect chemical cues in the environment and can signal the availability of food or the presence of a predator. Appropriate behavioral responses to these chemical cues are therefore important for organismal survival and can influence traits such as organismal life span and food consumption. However, understanding the genetic mechanisms underlying odor-guided behavior, correlated responses in other traits, and how these constrain or promote their evolution, remain an important challenge. Here, we performed artificial selection for attractive and aversive behavioral responses to four chemical compounds, two aromatics (4-ethylguaiacol and 4-methylphenol) and two esters (methyl hexanoate and ethyl acetate), for thirty generations. Artificial selection for odor-guided behavior revealed symmetrical responses to selection for each of the four chemical compounds. We then investigated whether selection for odor-guided behavior resulted in correlated responses in life history traits and/or food consumption. We found changes in food consumption upon selection for behavioral responses to aromatics. In many cases, lines selected for increased attraction to aromatics showed an increase in food consumption. We then performed RNA sequencing of lines selected for responses to 4-ethylguaiacol to identify candidate genes associated with odor-guided behavior and its impact on food consumption. We identified 91 genes that were differentially expressed among lines, many of which were associated with metabolic processes. RNAi-mediated knockdown of select candidate genes further supports their role in odor-guided behavior and/or food consumption. This study identifies novel genes underlying variation in odor-guided behavior and further elucidates the genetic mechanisms underlying the interrelationship between olfaction and feeding. The online version of this article (10.1186/s12864-017-4233-1) contains supplementary material, which is available to authorized users.
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DOI：
10.1101/lm.034363.114
发表时间：
2014-10
期刊：
Learning & memory (Cold Spring Harbor, N.Y.)
影响因子：
--
作者：
Guven-Ozkan T;Davis RL
通讯作者：
Davis RL
影响因子：
16.2
作者：
Dobritsa, AA;van der Goes van Naters, W;Carlson, JR
通讯作者：
Carlson, JR
影响因子：
3.3
作者：
Arya, Gunjan H.;Weber, Allison L.;Anholt, Robert R. H.
通讯作者：
Anholt, Robert R. H.
影响因子：
2.6
作者：
Garlapow ME;Everett LJ;Zhou S;Gearhart AW;Fay KA;Huang W;Morozova TV;Arya GH;Turlapati L;St Armour G;Hussain YN;McAdams SE;Fochler S;Mackay TF
通讯作者：
Mackay TF
影响因子：
48
作者：
Deshpande, Sonali A.;Carvalho, Gil B.;Amador, Ariadna;Phillips, Angela M.;Hoxha, Sany;Lizotte, Keith J.;Ja, William W.
通讯作者：
Ja, William W.