Genome-based construction of the metabolic pathways of Orientia tsutsugamushi and comparative analysis within the Rickettsiales order.
Genome-based construction of the metabolic pathways of Orientia tsutsugamushi and comparative analysis within the Rickettsiales order.
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DOI：
10.1155/2008/623145
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发表时间：
2008
影响因子：
--
通讯作者：
Cho NH
中科院分区：
文献类型：
--
作者：
Min CK;Yang JS;Kim S;Choi MS;Kim IS;Cho NH
恙虫病东方体是立克次体属的一种专性胞内细菌，是森林伤寒的病原体。最近，我们报道了恙虫病原虫具有独特的基因组结构，由高度重复的序列组成，这表明它可能为细胞内细菌的进化提供有价值的见解。在这里，我们利用基因组信息构建了恙虫病原虫的主要代谢途径，并与立克次体的其他成员进行了代谢基因和代谢途径的比较分析。虽然恙虫病原虫在该目的成员中拥有最大的基因组，主要是由于存在重复序列，但其代谢途径已经高度简化。总体上，恙虫病原虫的代谢途径与立克次体相似，但在碳水化合物代谢、TCA循环、细胞壁成分的合成以及运输系统等几个途径上存在显著差异。我们的结果将为恙虫病原虫的基因组后分析提供有用的指导，并有助于更好地了解这种细胞内病原体的毒力和生理学。
Orientia tsutsugamushi, the causative agent of scrub typhus, is an obligate intracellular bacterium that belongs to the order of Rickettsiales. Recently, we have reported that O. tsutsugamushi has a unique genomic structure, consisting of highly repetitive sequences, and suggested that it may provide valuable insight into the evolution of intracellular bacteria. Here, we have used genomic information to construct the major metabolic pathways of O. tsutsugamushi and performed a comparative analysis of the metabolic genes and pathways of O. tsutsugamushi with other members of the Rickettsiales order. While O. tsutsugamushi has the largest genome among the members of this order, mainly due to the presence of repeated sequences, its metabolic pathways have been highly streamlined. Overall, the metabolic pathways of O. tsutsugamushi were similar to Rickettsia but there were notable differences in several pathways including carbohydrate metabolism, the TCA cycle, and the synthesis of cell wall components as well as in the transport systems. Our results will provide a useful guide to the postgenomic analysis of O. tsutsugamushi and lead to a better understanding of the virulence and physiology of this intracellular pathogen.
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影响因子：
4.5
作者：
Dunning Hotopp JC;Lin M;Madupu R;Crabtree J;Angiuoli SV;Eisen JA;Seshadri R;Ren Q;Wu M;Utterback TR;Smith S;Lewis M;Khouri H;Zhang C;Niu H;Lin Q;Ohashi N;Zhi N;Nelson W;Brinkac LM;Dodson RJ;Rosovitz MJ;Sundaram J;Daugherty SC;Davidsen T;Durkin AS;Gwinn M;Haft DH;Selengut JD;Sullivan SA;Zafar N;Zhou L;Benahmed F;Forberger H;Halpin R;Mulligan S;Robinson J;White O;Rikihisa Y;Tettelin H
通讯作者：
Tettelin H
影响因子：
14.9
作者：
Ren Q;Chen K;Paulsen IT
通讯作者：
Paulsen IT
立克的立克氏菌的基因组序列鉴定出强制性细胞内寄生虫中的第一个假定的共轭质粒。
影响因子：
9.8
作者：
Ogata H;Renesto P;Audic S;Robert C;Blanc G;Fournier PE;Parinello H;Claverie JM;Raoult D
通讯作者：
Raoult D
影响因子：
14.9
作者：
Ren, QH;Kang, KH;Paulsen, IT
通讯作者：
Paulsen, IT
影响因子：
3.2
作者：
Audia, Jonathon P.;Winkler, Herbert H.
通讯作者：
Winkler, Herbert H.