Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets.
Histopathological, immunohistochemical, and ultrastructural evidence of spontaneous Senecavirus A-induced lesions at the choroid plexus of newborn piglets.
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DOI：
10.1038/s41598-017-16407-0
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发表时间：
2017-11-29
影响因子：
4.6
通讯作者：
Headley SA
中科院分区：
文献类型：
--
作者：
Oliveira TES;Michelazzo MMZ;Fernandes T;de Oliveira AG;Leme RA;Alfieri AF;Alfieri AA;Headley SA
流行性短暂新生儿损失（ETNL）是由塞内卡病毒a （SVA）引起的仔猪疾病，其传播方法和相关病变尚不明确。本研究通过检测新生仔猪的自发性感染，探讨了sva可能引起的病变。组织病理学显示移行上皮水肿变性，非化脓性脑膜脑炎，脉络丛炎和萎缩性肠炎。RT-PCR在所有评估的组织中鉴定出SVA，测序证实了这些结果。在所有器官的内皮组织和上皮组织中均观察到SVA阳性免疫反应。半薄层分析显示小肠顶端肠细胞空泡化，脉络膜丛内皮细胞球囊变性和坏死，非化脓性脉络膜丛炎。超薄评价显示顶端肠细胞水肿变性，CP开孔毛细血管内皮变性和坏死，室管膜细胞变性，病变内病毒颗粒相关。因此，SVA最初感染新生仔猪的顶端肠细胞，并可能通过CP进入大脑进入循环系统，首先产生最初的炎症反应，随后传播到脑部。因此，SVA可能采用肠-神经传播方式。
Epidemic Transient Neonatal Losses (ETNL) is a disease of piglets caused by Senecavirus A (SVA) in which the method of dissemination and associated lesions are not well-defined. This study investigated the possible SVA-induced lesions by examining spontaneous infections in newborn piglets. Histopathology revealed ballooning degeneration of transitional epithelium, nonsuppurative meningoencephalitis, plexus choroiditis, and atrophic enteritis. RT-PCR identified SVA in all tissues evaluated and sequencing confirmed these results. Positive immunoreactivity to SVA was observed in endothelial and epithelial tissues of all organs evaluated. Semithin analysis revealed vacuolization of apical enterocytes of the small intestine, balloon degeneration and necrosis of endothelial cells of the choroid plexus (CP) and nonsuppurative choroid plexitis. Ultrathin evaluation demonstrated hydropic degeneration of apical enterocytes, degeneration and necrosis of endothelium of CP fenestrated capillaries, degeneration of ependymocytes associated with intralesional viral particles. It is proposed that SVA initially infects apical enterocytes of newborn piglets and probably enters the circulatory system with entry to the brain via the CP, by first producing an initial inflammatory reaction, with subsequent encephalitic dissemination. Consequently, SVA probably uses an enteric-neurological method of dissemination.
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DOI：
10.1038/nrn3921
发表时间：
2015-08
期刊：
Nature reviews. Neuroscience
影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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