Involvement of microtubules and 10-nm filaments in the movement and positioning of nuclei in syncytia.
Involvement of microtubules and 10-nm filaments in the movement and positioning of nuclei in syncytia.
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DOI：
10.1083/jcb.83.2.320
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发表时间：
1979-11
期刊：
影响因子：
--
通讯作者：
Choppin PW
中科院分区：
文献类型：
--
作者：
Wang E;Cross RK;Choppin PW
以前的研究（Holmes, k.v.和P.W. Choppin）。中华医学杂志。124:501- 520；[j] .细胞生物学，39:526-543]表明，副流感病毒SV5感染幼鼠肾（BHK21-F）细胞可引起广泛的细胞融合，细胞核在合胞细胞质中迁移并排列成紧密排列的行，微管参与细胞核的运动和排列。微管、10nm细丝和含有肌动蛋白的微丝在这一过程中的作用已通过免疫荧光显微镜、特异性抗血清、延时电影摄影和电子显微镜进行了研究。在细胞融合过程中，来自许多细胞的微管和10nm细丝形成大束，这些大束位于细胞核之间。在这些区域没有检测到有组织的肌动蛋白纤维束，尽管在远离排列细胞核的区域观察到肌动蛋白纤维束。虽然秋水仙碱破坏微管并抑制核运动，但细胞松弛素B （CB； 20-50微克/毫升）不抑制细胞融合或核运动。然而，CB改变合胞体的形状，导致长丝状突起从中心区域延伸出来。当邻近细胞的这些过程接触时，发生融合，细胞核通过形成的通道迁移。电子显微镜和免疫荧光显微镜显示在这些过程中平行排列的微管束和10纳米细丝，但未检测到微细丝束。在这种高浓度（20微克/毫升）下，CB对微丝结构完整性的影响通过与重肌球蛋白相互作用的微丝消失而得到证实。环己亚胺（20微克/毫升）抑制蛋白质合成，但不影响细胞融合、微管和10nm丝束的形成或核迁移和排列；因此，不需要继续合成蛋白质。微管和10nm丝与细胞核迁移和排列的关联表明，微管和10nm丝在细胞内运动和细胞核位置中起着细胞骨架和产生力的作用。
Previous studies (Holmes, K.V., and P.W. Choppin. J. Exp. Med. 124:501- 520; J. Cell Biol. 39:526-543) showed that infection of baby hamster kidney (BHK21-F) cells with the parainfluenza virus SV5 causes extensive cell fusion, that nuclei migrate in the syncytial cytoplasm and align in tightly-packed rows, and that microtubules are involved in nuclear movement and alignment. The role of microtubules, 10-nm filaments, and actin-containing microfilaments in this process has been investigated by immunofluorescence microscopy using specific antisera, time-lapse cinematography, and electron microscopy. During cell fusion, micro tubules and 10-nm filaments from many cells form large bundles which are localized between rows of nuclei. No organized bundles of actin fibers were detected in these areas, although actin fibers were observed in regions away from the aligned nuclei. Although colchicine disrupts microtubules and inhibits nuclear movement, cytochalasin B (CB; 20-50 microgram/ml) does not inhibit cell fusion or nuclear movement. However, CB alters the shape of the syncytium, resulting in long filamentous processes extending from a central region. When these processes from neighboring cells make contact, fusion occurs, and nuclei migrate through the channels which are formed. Electron and immunofluorescence microscopy reveal bundles of microtubules and 10-nm filaments in parallel arrays within these processes, but no bundles of microfilaments were detected. The effect of CB on the structural integrity of microfilaments at this high concentration (20 microgram/ml) was demonstrated by the disappearance of filaments interacting with heavy meromyosin. Cycloheximide (20 microgram/ml) inhibits protein synthesis but does not affect cell fusion, the formation of microtubules and 10-nm filament bundles, or nuclear migration and alignment; thus, continued protein synthesis is not required. The association of microtubules and 10-nm filaments with nuclear migration and alignment suggests that microtubules and 10-nm filaments are two components in a system which serves both cytoskeletal and force-generating functions in intracellular movement and position of nuclei.
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影响因子：
7.8
作者：
HOLMES, KV;CHOPPIN, PW
通讯作者：
CHOPPIN, PW
影响因子：
3.7
作者：
ABERCROMBIE, M;HEAYSMAN, JE;PEGRUM, SM
通讯作者：
PEGRUM, SM
影响因子：
15.3
作者：
HOLMES, KV;CHOPPIN, PW
通讯作者：
CHOPPIN, PW
影响因子：
64.5
作者：
GORDON, WE;BUSHNELL, A;BURRIDGE, K
通讯作者：
BURRIDGE, K
DOI：
10.1007/bf00330921
发表时间：
1971-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
BLUEMINK, JG
通讯作者：
BLUEMINK, JG