Effect of iron oxide and gold nanoparticles on bacterial growth leading towards biological application.
Effect of iron oxide and gold nanoparticles on bacterial growth leading towards biological application.
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DOI：
10.1186/1477-3155-9-34
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发表时间：
2011-08-23
影响因子：
10.2
通讯作者：
Sarkar K
中科院分区：
文献类型：
--
作者：
Chatterjee S;Bandyopadhyay A;Sarkar K
纳米金属氧化物和金代表着一类新的重要材料，越来越多地被开发用于研究和与健康有关的活动。生物系统是极其重要的，需要对无机纳米颗粒对细胞生长和功能的影响有基本的了解。本研究旨在探讨氧化铁(Fe3O4)、金(Au)纳米粒子对大肠杆菌细胞生长的影响，并试图通过对Au纳米粒子进行功能化来引导其进一步的生物应用。制备了Fe3O4和Au纳米粒子，并用透射电子显微镜(TEM)和动态光散射(DLS)进行了表征。初步的生长分析数据表明，氧化铁纳米颗粒对大肠杆菌的抑制作用呈浓度依赖关系，而纳米金颗粒则没有这种活性。然而，相差显微镜研究清楚地表明，Fe3O4和Au纳米粒子的作用都延伸到了细胞分裂的水平，这明显地表现为细菌细胞长度的突然增加。在显微分析的基础上，制备了谷胱甘肽功能化的金纳米颗粒，并将其作为载体在细菌细胞内转运。总之，研究表明，金属纳米颗粒与细菌在细胞水平上存在相互作用，可用于有益的生物应用，但值得注意的是，它也具有产生生态毒性的潜力，挑战了纳米颗粒的生态友好性质。
Nanoparticle-metal oxide and gold represents a new class of important materials that are increasingly being developed for use in research and health related activities. The biological system being extremely critical requires the fundamental understanding on the influence of inorganic nanoparticles on cellular growth and functions. Our study was aimed to find out the effect of iron oxide (Fe3O4), gold (Au) nanoparticles on cellular growth of Escherichia coli (E. coli) and also try to channelize the obtained result by functionalizing the Au nanoparticle for further biological applications. Fe3O4 and Au nanoparticles were prepared and characterized using Transmission electron microscopy (TEM) and Dynamic Light Scattering (DLS). Preliminary growth analysis data suggest that the nanoparticles of iron oxide have an inhibitory effect on E. coli in a concentration dependant manner, whereas the gold nanoparticle directly showed no such activity. However the phase contrast microscopic study clearly demonstrated that the effect of both Fe3O4 and Au nanoparticle extended up to the level of cell division which was evident as the abrupt increase in bacterial cell length. The incorporation of gold nanoparticle by bacterial cell was also observed during microscopic analysis based on which glutathione functionalized gold nanoparticle was prepared and used as a vector for plasmid DNA transport within bacterial cell. Altogether the study suggests that there is metal nanoparticle-bacteria interaction at the cellular level that can be utilized for beneficial biological application but significantly it also posses potential to produce ecotoxicity, challenging the ecofriendly nature of nanoparticles.
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影响因子：
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作者：
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