Enhanced Intracranial Microdialysis by Reduction of Traumatic Penetration Injury at the Probe Track.
Enhanced Intracranial Microdialysis by Reduction of Traumatic Penetration Injury at the Probe Track.
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通过减少探头轨道的创伤性穿透损伤来增强颅内微透析。
DOI：
10.1021/acschemneuro.5b00331
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发表时间：
2016
影响因子：
5
通讯作者：
Michael,AdrianC
中科院分区：
文献类型：
--
作者：
Varner,ErikaL;Jaquins-Gerstl,Andrea;Michael,AdrianC
微透析通过体内颅内化学监测提供了对化学神经科学的深入了解。然而，植入微透析探针会导致探针轨道处脑组织的创伤性穿透损伤（TPI）。伏安法和组织化学成像清楚地记录了TPI，但TPI是一个缺点，因为它会干扰脑透析液样本来源的确切组织。我们的目标是减少（如果不是最终消除）TPI及其对神经化学监测的有害影响。在这里，我们证明了探头植入后5天的等待期与低微摩尔浓度地塞米松的连续后透析相结合，大大降低了TPI。我们的方法，以减少TPI恢复正常诱发多巴胺释放活动的组织附近的微透析探针，使诱发多巴胺释放在探针出口到定量协议与诱发多巴胺释放旁边的探针，恢复正常的酪氨酸羟化酶和多巴胺转运蛋白的免疫反应性探针轨道附近，并大大抑制神经胶质细胞激活和疤痕附近的探针轨道。TPI的降低和探头轨迹附近正常诱发多巴胺释放活动的恢复似乎是由于地塞米松的抗炎作用。
Microdialysis provides deep insight into chemical neuroscience by enabling in vivo intracranial chemical monitoring. Nevertheless, implanting a microdialysis probe causes a traumatic penetration injury (TPI) of brain tissue at the probe track. The TPI, which is clearly documented by voltammetry and histochemical imaging, is a drawback because it perturbs the exact tissue from which the brain dialysate samples are derived. Our goal is to reduce, if not eventually eliminate, the TPI and its detrimental effects on neurochemical monitoring. Here, we demonstrate that combining a 5-day wait period after probe implantation with the continuous retrodialysis of a low-micromolar concentration of dexamethasone vastly reduces the TPI. Our approach to reducing the TPI reinstates normal evoked dopamine release activity in the tissue adjacent to the microdialysis probe, brings evoked dopamine release at the probe outlet into quantitative agreement with evoked dopamine release next to the probe, reinstates normal immunoreactivity for tyrosine hydroxylase and the dopamine transporter near the probe track, and greatly suppresses glial activation and scaring near the probe track. This reduction of the TPI and reinstatement of normal evoked dopamine release activity adjacent to the probe track appears to be due to dexamethasone’s anti-inflammatory actions.
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DOI：
10.1016/s0002-9440(10)64686-x
发表时间：
2001-06
期刊：
The American journal of pathology
影响因子：
--
作者：
F. Zhou;X. Zhu;R. Castellani;R. Stimmelmayr;George Perry;M. Smith;K. Drew
通讯作者：
F. Zhou;X. Zhu;R. Castellani;R. Stimmelmayr;George Perry;M. Smith;K. Drew
糖皮质激素类似物地塞米松改变皮质-皮质下区域内多巴胺受体和脑啡肽的表达和分布。
影响因子：
2.7
作者：
F. Iasevoli;L. Aloj;G. Latte;L. Avvisati;F. Marmo;C. Tomasetti;E. Buonaguro;C. Simeoli;R. Pivonello;A. Colao;A. de Bartolomeis
通讯作者：
A. de Bartolomeis
影响因子：
7.4
作者：
Jaquins-Gerstl, Andrea;Shu, Zhan;Zhang, Jing;Liu, Yansheng;Weber, Stephen G.;Michael, Adrian C.
通讯作者：
Michael, Adrian C.
影响因子：
12.7
作者：
BENVENISTE, H;DIEMER, NH
通讯作者：
DIEMER, NH
影响因子：
3.4
作者：
Lecca, D;Cacciapaglia, F;Di Chiara, G
通讯作者：
Di Chiara, G