High-resolution magic angle spinning NMR spectroscopy of human osteoarthritic cartilage.
High-resolution magic angle spinning NMR spectroscopy of human osteoarthritic cartilage.
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DOI：
10.1002/nbm.1769
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发表时间：
2012-04
影响因子：
2.9
通讯作者：
Li, Xiaojuan
中科院分区：
文献类型：
--
作者：
Shet, Keerthi;Siddiqui, Sarmad M.;Yoshihara, Hikari;Kurhanewicz, John;Ries, Michael;Li, Xiaojuan
骨关节炎（OA）是一种关节退行性疾病，导致软骨生化成分的变化。过去已经进行了使用高分辨率核磁共振光谱来研究猪、狗和牛软骨的生化组成的研究。在本研究中，采用11.7 T的高分辨率旋角（HRMAS）核磁共振光谱来表征代谢物，并检测非OA健康尸体膝盖和严重OA患者全膝关节置换术时采集的样本的膝关节软骨光谱特征的差异。在健康和OA样本之间观察到丙氨酸（1.47 ppm）、n -乙酰基（2.04 ppm）、胆碱（3.25 ppm）和甘氨酸（3.55 ppm）代谢物水平的统计显著差异。研究结果表明，在骨性关节炎晚期，n -乙酰基共振强度下降。观察到1H HR-MAS NMR光谱测量的n -乙酰基水平与GAG测定的同一软骨标本中总蛋白多糖含量呈正相关。这表明n -乙酰基可以作为OA疾病进展的重要生物标志物。OA中丙氨酸浓度的降低可能是由于随着疾病进展胶原框架的降解以及从软骨转运到滑膜腔的降解产物的最终丢失。
Osteoarthritis (OA) is a degenerative disease of the joint and results in changes in the biochemical composition of cartilage. Studies have been undertaken in the past that have used high resolution NMR spectroscopy to study the biochemical composition of porcine, canine and bovine cartilage. In this study high resolution magical angle spinning (HRMAS) NMR spectroscopy at 11.7 T has been used to characterize metabolites and detect differences in the spectral signature of human knee articular cartilage from non-OA healthy cadaver knees and samples acquired from severe OA patients at the time of total knee replacement surgery. A statistically significant difference in the alanine (1.47 ppm), N-acetyl (2.04 ppm), choline (3.25 ppm) and glycine (3.55 ppm) metabolite levels is observed between healthy and OA specimens. The results of the study indicate that a decrease in the intensity of N-acetyl resonance occurs in later stages of OA. A positive correlation of the N-acetyl levels as measured by 1H HR-MAS NMR spectroscopy with the total proteoglycan content in the same cartilage specimens as measured by the GAG assay was observed. This indicates that N-acetyl can serve as an important bio-marker of OA disease progression. A decrease in the alanine concentration in OA may be attributed to the degradation of the collagen framework with disease progression and eventual loss of the degradation products that are transported from cartilage into the synovial cavity.
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影响因子：
3.3
作者：
Swanson, Mark G.;Keshari, Kayvan R.;Tabatabai, Z. Laura;Simko, Jeffry P.;Shinohara, Katsuto;Carroll, Peter R.;Zektzer, Andrew S.;Kurhanewicz, John
通讯作者：
Kurhanewicz, John
影响因子：
2.9
作者：
Opstad, Kirstie S.;Bel, B. Anthony;Howe, Franklyn A.
通讯作者：
Howe, Franklyn A.
影响因子：
2.9
作者：
RICHMOND, ME;DELUCA, S;SILBERT, JE
通讯作者：
SILBERT, JE
影响因子：
3.3
作者：
Swanson, MG;Zektzer, AS;Kurhanewicz, J
通讯作者：
Kurhanewicz, J
影响因子：
3.3
作者：
Keshari, KR;Zektzer, AS;Kurhanewicz, J
通讯作者：
Kurhanewicz, J