Intrastrain and interstrain genetic variation within a paralogous gene family in Chlamydia pneumoniae.
Intrastrain and interstrain genetic variation within a paralogous gene family in Chlamydia pneumoniae.
复制标题
肺炎衣原体中寄生虫基因家族中的腹腔内和遗传变异。
DOI：
10.1186/1471-2180-2-38
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发表时间：
2002-12-02
期刊：
影响因子：
4.2
通讯作者：
Rockey, Daniel D.
中科院分区：
文献类型：
--
作者：
Viratyosin, Wasna;Campbell, Lee Ann;Kuo, Cho-Chou;Rockey, Daniel D.
肺炎衣原体引起人类呼吸道疾病，最近被认为与动脉粥样硬化有关。分析了最近发表的三种C.通过对肺炎克雷伯氏菌基因组的分析，鉴定出一个新的基因家族（Cpn 1054家族），该家族由11个预测的基因和基因片段组成。每个成员编码一种多肽，该多肽具有定位于包涵体膜的蛋白质的疏水结构域特征。在已发表的基因组序列中对该基因家族的比较分析提供了证据，表明在这一单一的旁系同源基因集合中，多个水平的遗传变异是明显的。移码突变被发现，导致截短的基因产物和假基因，不同的菌株。该家族中的几个基因在预测的编码序列的上游或末端5'端内含有多胞嘧啶（polyC）区。polyC延伸的长度在三个基因组中的旁系同源基因之间和单个基因内变化。12株C.使用肺炎临床分离株来确定Cpn 1054基因家族的变异程度。这些研究表明，在几个基因座上，菌株之间和菌株内都存在序列变异性。特别是，与不同Cpn 1054基因家族成员相关的polyC束长度的变化在每个测试的C中是常见的。肺炎分离株。在这个新描述的基因家族中鉴定的变异性可能调节C. pneumoniae人群
Chlamydia pneumoniae causes human respiratory diseases and has recently been associated with atherosclerosis. Analysis of the three recently published C. pneumoniae genomes has led to the identification of a new gene family (the Cpn 1054 family) that consists of 11 predicted genes and gene fragments. Each member encodes a polypeptide with a hydrophobic domain characteristic of proteins localized to the inclusion membrane. Comparative analysis of this gene family within the published genome sequences provided evidence that multiple levels of genetic variation are evident within this single collection of paralogous genes. Frameshift mutations are found that result in both truncated gene products and pseudogenes that vary among isolates. Several genes in this family contain polycytosine (polyC) tracts either upstream or within the terminal 5' end of the predicted coding sequence. The length of the polyC stretch varies between paralogous genes and within single genes in the three genomes. Sequence analysis of genomic DNA from a collection of 12 C. pneumoniae clinical isolates was used to determine the extent of the variation in the Cpn 1054 gene family. These studies demonstrate that sequence variability is present both among strains and within strains at several of the loci. In particular, changes in the length of the polyC tract associated with the different Cpn 1054 gene family members are common within each tested C. pneumoniae isolate. The variability identified within this newly described gene family may modulate either phase or antigenic variation and subsequent physiologic diversity within a C. pneumoniae population.
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