Translationally controlled tumor protein GmTCTP interacts with GmCDPKSK5 in response to high temperature and humidity stress during soybean seed development
Translationally controlled tumor protein GmTCTP interacts with GmCDPKSK5 in response to high temperature and humidity stress during soybean seed development
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翻译控制的肿瘤蛋白 GmTCTP 与 GmCDPKSK5 相互作用以响应大豆种子发育过程中的高温和潮湿胁迫
DOI：
10.1007/s10725-017-0250-y
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发表时间：
2017-03
影响因子：
4.2
通讯作者：
Ma Hao
中科院分区：
文献类型：
--
作者：
Wang Shuang;Tao Yuan;Zhou Yali;Niu Juan;Shu Yingjie;Yu Xingwang;Liu Sushuang;Chen Ming;Gu Weihong;Ma Hao
大豆种子发育过程中易受高温高湿胁迫的影响，导致田间采前种子劣变。许多基因被发现对压力做出反应。在前期蛋白质组学研究的基础上，从大豆种子中分离到一个高温高压反应基因GmCDPKSK 5，该基因编码一个胞质和膜相关蛋白，属于CDPK家族的第I组。利用酵母双杂交技术（Y2H）从大豆种子cDNA文库中筛选到一个与GmCDPKSK 5相互作用的蛋白GmTCTP。GmCDPKSK 5和GmTCTP之间的相互作用进一步验证了使用双分子荧光互补和GST下拉分析。HTH胁迫诱导了大豆种子中GmCDPKSK 5和GmTCTP的表达水平。这些结果表明，GmCDPKSK 5和GmTCTP在大豆发育过程中相互作用，可能参与了大豆对HTH胁迫的反应。
The developing seed of soybean is susceptible to high temperature and humidity (HTH) stress, resulting in pre-harvest seed deterioration in the field. Many genes are found to respond to the stress. Based on our previous proteomics study, an HTH-responsive gene,GmCDPKSK5, was isolated from soybean seed.GmCDPKSK5encodes a cytoplasm- and membrane-associated protein, which belongs to Group I of the CDPK family. By yeast two-hybrid (Y2H) from soybean seed cDNA library, GmTCTP was screened as a GmCDPKSK5-interacting protein. The interaction between GmCDPKSK5 and GmTCTP was further verified using bimolecular fluorescence complementation and GST pull down assays. Expression levels of bothGmCDPKSK5andGmTCTPwere induced by HTH stress in soybean seed. Our results indicated that GmCDPKSK5 and GmTCTP interact with each other and may function in responses to HTH stress in soybean developing seed.
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