Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells.
Analyses of Avascular Mutants Reveal Unique Transcriptomic Signature of Non-conventional Endothelial Cells.
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DOI：
10.3389/fcell.2020.589717
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发表时间：
2020
影响因子：
5.5
通讯作者：
Jin SW
中科院分区：
文献类型：
--
作者：
Pak B;Schmitt CE;Choi W;Kim JD;Han O;Alsiö J;Jung DW;Williams DR;Coppieters W;Stainier DYR;Jin SW
内皮细胞似乎来自不同的祖细胞。然而，在何种程度上他们的发展起源有助于定义其细胞和分子特征仍然是未知的。在这里，我们报告说，从尾芽出现的内皮细胞的一个子集具有独特的分子特征，使他们除了刻板的侧板中胚层（LPM）衍生的内皮细胞。谱系追踪显示，这些尾芽衍生的内皮细胞出现在中期体节发生阶段，并且令人惊讶地不需要Npas 4l或Etsrp功能，这表明它们具有不同的时空起源，并且由不同的分子机制调节。微阵列和单细胞RNA-seq分析显示，体节发生和神经发生相关的转录本在这些尾芽衍生的内皮细胞中过度表达，表明它们具有独特的转录组学特征。两者合计，我们的研究结果进一步揭示了内皮细胞的多样性，就其发展的起源和分子特性，并提供令人信服的证据表明，内皮细胞的分子特征可能反映其独特的发展历史。
Endothelial cells appear to emerge from diverse progenitors. However, to which extent their developmental origin contributes to define their cellular and molecular characteristics remains largely unknown. Here, we report that a subset of endothelial cells that emerge from the tailbud possess unique molecular characteristics that set them apart from stereotypical lateral plate mesoderm (LPM)-derived endothelial cells. Lineage tracing shows that these tailbud-derived endothelial cells arise at mid-somitogenesis stages, and surprisingly do not require Npas4l or Etsrp function, indicating that they have distinct spatiotemporal origins and are regulated by distinct molecular mechanisms. Microarray and single cell RNA-seq analyses reveal that somitogenesis- and neurogenesis-associated transcripts are over-represented in these tailbud-derived endothelial cells, suggesting that they possess a unique transcriptomic signature. Taken together, our results further reveal the diversity of endothelial cells with respect to their developmental origin and molecular properties, and provide compelling evidence that the molecular characteristics of endothelial cells may reflect their distinct developmental history.
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影响因子：
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作者：
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作者：
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通讯作者：
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