Association of cytokeratin 17 expression with differentiation in oral squamous cell carcinoma.
Association of cytokeratin 17 expression with differentiation in oral squamous cell carcinoma.
复制标题
DOI：
10.1007/s00432-012-1202-6
复制
发表时间：
2012-08
影响因子：
3.6
通讯作者：
Nakamura, Seiji
中科院分区：
文献类型：
--
作者：
Kitamura, Ryoji;Toyoshima, Takeshi;Tanaka, Hideaki;Kawano, Shintaro;Kiyosue, Takahiro;Matsubara, Ryota;Goto, Yuichi;Hirano, Mitsuhiro;Oobu, Kazunari;Nakamura, Seiji
关键词：
本研究的目的是比较细胞角蛋白(CK)17和CK13在口腔鳞状细胞癌(OSCC)和白斑中的表达情况，探讨CK17与OSCC分化的关系。应用免疫组织化学方法检测105例口腔鳞癌和108例口腔白斑组织中CK17和CK13的表达。将CK的表达与临床病理变量进行相关性分析。实时荧光定量RT-PCR检测5种口腔鳞癌细胞系(HSC-2、HSC-3、SAS、SQUU-A、SQUU-B)中CK17基因的过表达水平。在105例口腔鳞癌中，101例(96.2%)表达CK17，3例(2.9%)表达CK13。CK17在高分化口腔鳞癌中的表达显著高于中/低分化口腔鳞癌(P<0.01)。在34例增生性白斑中19例(55.9%)和74例增生性白斑中36例(48.6%)CK17在增生性白斑中显著表达(P<0.01)。34例异型增生中11例(32.4%)和74例增生性白斑中52例(70.3%)CK13在增生性白斑中显著表达(P<0.01)。CK17mRNA在HSC-2中的相对表达显著高于HSC-3和SAS(P<0.05)。此外，CK17mRNA在SQUU-A组的相对表达显著高于SQUU-B组(P<0.05)。CK17的表达可能与口腔鳞癌的分化程度和恶性程度有关。CK17/CK13的结合模式可能是一个合适的恶变标志物。
The aim of this study was to confirm the expression profile of cytokeratin (CK)17 in comparison with that of CK13 in oral squamous cell carcinoma (OSCC) and leukoplakia and to clarify an association of CK17 with the OSCC differentiation. The expression of CK17 and CK13 was immunohistochemically examined in 105 patients with OSCC and 108 patients with leukoplakia. A correlation of CK expression with clinicopathological variables was carried out. The over-expression levels of CK17 mRNA were analyzed by real-time RT-PCR in 5 OSCC cell lines (HSC-2, HSC-3, SAS, SQUU-A, SQUU-B). CK17 and CK13 were detected in 101 (96.2 %) and three (2.9 %) of the 105 OSCCs, respectively. CK17 was significantly expressed in well-differentiated OSCC compared to moderately/poorly differentiated OSCC (p < 0.01). As detected in 19 of the 34 dysplastic leukoplakias (55.9 %) and 36 of the 74 hyperplastic leukoplakias (48.6 %), CK17 was significantly expressed in dysplastic leukoplakias (p < 0.01). As detected in 11 of the 34 dysplastic (32.4 %) and 52 of the 74 hyperplastic leukoplakias (70.3 %), CK13 was significantly expressed in hyperplastic leukoplakias (p < 0.01). The relative expression of CK17 mRNA in HSC-2 was significantly higher than in HSC-3 and SAS (p < 0.05). Moreover, the relative expression of CK17 mRNA in SQUU-A was significantly higher than in SQUU-B (p < 0.05). CK17 expression could be associated with the differentiation and the malignancy of OSCC. A combination pattern of CK17/CK13 might be a suitable marker of malignant transformation.
登录
查看更多内容
影响因子：
3.3
作者：
Bloor, BK;Seddon, SV;Morgan, PR
通讯作者：
Morgan, PR
影响因子：
3.3
作者：
Toyoshima, T.;Koch, F.;Wagner, W.
通讯作者：
Wagner, W.
影响因子：
3.6
作者：
KAWAHARA, E;IMAI, K;NAKANISHI, I
通讯作者：
NAKANISHI, I
影响因子：
3.3
作者：
VAIDYA, MM;BORGES, AM;BHISEY, AN
通讯作者：
BHISEY, AN
影响因子：
3.3
作者：
VIGNESWARAN, N;PETERS, KP;HANEKE, E
通讯作者：
HANEKE, E