Endometrial regenerative cells: a novel stem cell population.
Endometrial regenerative cells: a novel stem cell population.
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子宫内膜再生细胞：一种新型的干细胞群体。
DOI：
10.1186/1479-5876-5-57
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发表时间：
2007-11-15
影响因子：
7.4
通讯作者：
Riordan, Neil H.
中科院分区：
文献类型：
--
作者：
Meng, Xiaolong;Ichim, Thomas E.;Zhong, Jie;Rogers, Andrea;Yin, Zhenglian;Jackson, James;Wang, Hao;Ge, Wei;Bogin, Vladimir;Chan, Kyle W.;Thebaud, Bernard;Riordan, Neil H.
血管生成是月经周期增生期子宫内膜的重要组成部分。因此，我们假设存在干细胞样群，并且可以从月经血中分离出来。从月经血中采集的单个核细胞含有贴壁细胞亚群，可在组织培养中保持>；68倍体，并保留CD9、CD29、CD41a、CD44、CD59、CD73、CD90和CD105的表达，无核型异常。细胞增殖率明显高于对照脐带间充质干细胞，每19.4小时倍增一倍。这些细胞，我们称之为子宫内膜再生细胞(ERC)，能够分化为9个谱系：心肌细胞、呼吸道上皮细胞、神经细胞、肌细胞、内皮细胞、胰腺、肝脏、脂肪细胞和成骨细胞。此外，ERC产生的MMP3、MMP10、GM-CSF、血管生成素-2和PDGF-BB的水平比两个对照脐带血来源的间充质干细胞株高10-100,000倍。鉴于这种细胞群的提取容易和多能性，我们建议ERC作为当前干细胞来源的一种新的替代方案。
Angiogenesis is a critical component of the proliferative endometrial phase of the menstrual cycle. Thus, we hypothesized that a stem cell-like population exist and can be isolated from menstrual blood. Mononuclear cells collected from the menstrual blood contained a subpopulation of adherent cells which could be maintained in tissue culture for >68 doublings and retained expression of the markers CD9, CD29, CD41a, CD44, CD59, CD73, CD90 and CD105, without karyotypic abnormalities. Proliferative rate of the cells was significantly higher than control umbilical cord derived mesenchymal stem cells, with doubling occurring every 19.4 hours. These cells, which we termed "Endometrial Regenerative Cells" (ERC) were capable of differentiating into 9 lineages: cardiomyocytic, respiratory epithelial, neurocytic, myocytic, endothelial, pancreatic, hepatic, adipocytic, and osteogenic. Additionally, ERC produced MMP3, MMP10, GM-CSF, angiopoietin-2 and PDGF-BB at 10–100,000 fold higher levels than two control cord blood derived mesenchymal stem cell lines. Given the ease of extraction and pluripotency of this cell population, we propose ERC as a novel alternative to current stem cells sources.
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