Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens.
Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens.
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鸡艾美耳球虫共同抗原多表位疫苗的保护效果。
DOI：
10.1186/s13567-023-01253-y
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发表时间：
2023-12-13
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
临床上的禽球虫病通常是由几种艾美耳球虫共同感染引起的。重组蛋白和DNA疫苗在控制球虫病方面显示出了希望。在此基础上，编码来自不同艾美耳球虫物种的多个表位的DNA疫苗可以提供针对合并感染的广泛保护。在这项研究中，我们设计了一个融合基因片段，14 EGT，含有浓缩的T细胞表位从四个共同抗原的艾美耳球虫物种（14-3-3，延伸因子2，甘油醛-3-磷酸脱氢酶，和转氢酶）。构建了多表位DNA疫苗pVAX 1 - 14 EGT和重组蛋白疫苗pET-32 a-14 EGT（r14 EGT）。随后，在接种疫苗的鸡中测量细胞和体液免疫应答。还进行了疫苗接种-攻毒试验，其中用14 EGT制剂对禽类进行疫苗接种，随后暴露于单个或多个艾美耳球虫属物种，以评价疫苗的保护效力。根据结果，用14 EGT制剂接种有效地增加了CD 4+和CD 8 + T细胞的比例以及Th 1和Th 2标志性细胞因子的水平。血清IgG抗体水平也显著升高。动物接种试验显示，与对照组相比，肠道病变、体重减轻和卵囊排出量减轻。发现制剂对单一艾美耳球虫属物种具有中等有效性，抗球虫指数（ACI）范围为160至180。然而，在用多种艾美耳球虫属物种攻击后，14 EGT制剂提供的保护不令人满意，ACI值为142.18和146.41。总的来说，这些结果表明，编码来自艾美耳球虫寄生虫的共同抗原的T细胞表位的多表位疫苗可能是控制禽球虫病的潜在和有效的策略。在线版本包含补充材料，可通过10.1186/s13567-023-01253-y获得。
Clinical avian coccidiosis is typically caused by coinfection with several Eimeria species. Recombinant protein and DNA vaccines have shown promise in controlling coccidiosis. On this basis, DNA vaccines that encode multiple epitopes from different Eimeria species may provide broad protection against coinfections. In this study, we designed a fusion gene fragment, 14EGT, that contained concentrated T-cell epitopes from four common antigens of Eimeria species (14-3-3, elongation factor 2, glyceraldehyde-3-phosphate dehydrogenase, and transhydrogenase). The multiepitope DNA vaccine pVAX1-14EGT and recombinant protein vaccine pET-32a-14EGT (r14EGT) were then created based on the 14EGT fragment. Subsequently, cellular and humoral immune responses were measured in vaccinated chickens. Vaccination-challenge trials were also conducted, where the birds were vaccinated with the 14EGT preparations and later exposed to single or multiple Eimeria species to evaluate the protective efficacy of the vaccines. According to the results, vaccination with 14EGT preparations effectively increased the proportions of CD4+ and CD8+ T cells and the levels of Th1 and Th2 hallmark cytokines. The levels of serum IgG antibodies were also significantly increased. Animal vaccination trials revealed alleviated enteric lesions, weight loss, and oocyst output compared to those of the control groups. The preparations were found to be moderately effective against single Eimeria species, with the anticoccidial index (ACI) ranging from 160 to 180. However, after challenge with multiple Eimeria species, the protection provided by the 14EGT preparations was not satisfactory, with ACI values of 142.18 and 146.41. Collectively, the results suggest that a multiepitope vaccine that encodes the T-cell epitopes of common antigens derived from Eimeria parasites could be a potential and effective strategy to control avian coccidiosis. The online version contains supplementary material available at 10.1186/s13567-023-01253-y.
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影响因子：
4
作者：
Du, AF;Wang, SH
通讯作者：
Wang, SH
影响因子：
3.6
作者：
HOPP, TP;WOODS, KR
通讯作者：
WOODS, KR
影响因子：
2.6
作者：
Constantinoiu, CC;Lillehoj, HS;Baba, E
通讯作者：
Baba, E
DOI：
10.1007/s12639-017-0927-1
发表时间：
2017-12-01
期刊：
Journal of parasitic diseases : official organ of the Indian Society for Parasitology
影响因子：
--
作者：
Huang, Yueyue;Ruan, Xiangchun;Zeng, Minghua
通讯作者：
Zeng, Minghua
影响因子：
5.2
作者：
Chen C;Tian D;Su J;Liu X;Shah MAA;Li X;Xu L;Yan R;Song X
通讯作者：
Song X