Role of exogenous abscisic acid in freezing tolerance of mangrove Kandelia obovata under natural frost condition at near 32(°)N.
Role of exogenous abscisic acid in freezing tolerance of mangrove Kandelia obovata under natural frost condition at near 32(°)N.
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DOI：
10.1186/s12870-022-03990-2
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发表时间：
2022-12-19
影响因子：
5.3
通讯作者：
Chen, Qiuxia
中科院分区：
文献类型：
--
作者：
Liu, Xing;Lu, Xiang;Yang, Sheng;Liu, Yu;Wang, Wenqing;Wei, Xin;Ji, Hongjiu;Zhang, Bo;Xin, Wenzhen;Wen, Junxiu;Wang, Jinwang;Chen, Qiuxia
关键词：
红树林在热带和亚热带滨海湿地具有重要的生态、社会和经济功能。秋茄（Kandelia obovata）是红树林中抗寒性最强的树种，分布于三亚（18° 12′ N）至温州（28° 20′ N）一带。在这里，我们探讨了时间变化的生理状态和转录组分析K。研究了在~ 32 oN的自然霜冻条件下，不同浓度的外源脱落酸（阿坝）对银杉抗寒性的影响，以及外源ABA对银杉抗寒性的影响。可溶性糖（SS）和脯氨酸（Pro）在低温胁迫下起作用，其中SS对K的作用更大。obovata。低温诱导的DEGs表达上调与糖代谢有关，如淀粉和蔗糖代谢、氨基糖和核苷酸糖代谢。值得注意的是，KEGG富集的前2条途径是苯丙素类生物合成和类黄酮生物合成。在抗氧化系统中，POD和CAT共同作用去除过氧化氢，CAT的作用更大。低温和阿坝诱导表达的DEGs主要集中在精氨酸和脯氨酸代谢、淀粉和蔗糖代谢以及过氧化物酶体中。阿坝诱导P5 CS和P5 CR的表达，但抑制ProDH的表达，这可能有助于Pro的积累。低温胁迫期间叶片丙二醛（MDA）含量变化不显著（P > 0.05），表明叶面喷施阿坝能有效缓解低温胁迫对K. ABA通过激活抗氧化酶的活性和增加渗透调节物质Pro的积累来抑制倒卵花幼苗的生长，其效果与阿坝浓度成正比。本研究加深了对K.在田间水平上研究了obovata对自然霜冻条件和外源阿坝的响应，为在高纬度地区扩大红树林人工林种植以及开发滨海景观提供了理论依据。在线版本包含补充材料，可通过10.1186/s12870-022-03990-2获得。
Mangroves possess substantial ecological, social, and economic functions in tropical and subtropical coastal wetlands. Kandelia obovata is the most cold-resistance species among mangrove plants, with a widespread distribution in China that ranges from Sanya (18° 12′ N) to Wenzhou (28° 20′ N). Here, we explored the temporal variations in physiological status and transcriptome profiling of K. obovata under natural frost conditions at ~ 32oN, as well as the positive role of exogenous abscisic acid (ABA) in cold resistance. The soluble sugar (SS) and proline (Pro) functioned under freezing stress, of which SS was more important for K. obovata. Consistently, up-regulated DEGs responding to low temperature were significantly annotated to glycometabolism, such as starch and sucrose metabolism and amino sugar and nucleotide sugar metabolism. Notably, the top 2 pathways of KEGG enrichment were phenylpropanoid biosynthesis and flavonoid biosynthesis. For the antioxidant system, POD in conjunction with CAT removed hydrogen peroxide, and CAT appeared to be more important. The up-regulated DEGs responding to low temperature and ABA were also found to be enriched in arginine and proline metabolism, starch and sucrose metabolism, and peroxisome. Moreover, ABA triggered the expression of P5CS and P5CR, but inhibited the ProDH expression, which might contribute to Pro accumulation. Interestingly, there was no significant change in malondialdehyde (MDA) content during the cold event (P > 0.05), suggesting foliar application of ABA effectively alleviated the adverse effects of freezing stress on K. obovata by activating the antioxidant enzyme activity and increasing osmolytes accumulation, such as Pro, and the outcome was proportional to ABA concentration. This study deepened our understanding of the physiological characters and molecular mechanisms underlying the response of K. obovata to natural frost conditions and exogenous ABA at the field level, which could provide a sound theoretical foundation for expanding mangroves plantations in higher latitudes, as well as the development coastal landscape. The online version contains supplementary material available at 10.1186/s12870-022-03990-2.
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影响因子：
1.9
作者：
Huang, Xing;Chen, Ming-Hui;Wu, Jian-Ming
通讯作者：
Wu, Jian-Ming
影响因子：
7.4
作者：
Gilmour, SJ;Sebolt, AM;Thomashow, MF
通讯作者：
Thomashow, MF
DOI：
10.1073/pnas.1315800111
发表时间：
2014-01-14
影响因子：
11.1
作者：
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Feller, Ilka C.
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影响因子：
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影响因子：
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作者：
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