Promotion of articular cartilage matrix vesicle mineralization by type I collagen.
Promotion of articular cartilage matrix vesicle mineralization by type I collagen.
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DOI：
10.1002/art.23762
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发表时间：
2008-09
影响因子：
--
通讯作者：
Rosenthal AK
中科院分区：
文献类型：
--
作者：
Jubeck B;Gohr C;Fahey M;Muth E;Matthews M;Mattson E;Hirschmugl C;Rosenthal AK
焦磷酸钙二水合物（CPPD）和碱性磷酸钙（BCP）晶体出现在高达60%的骨关节炎关节，并预测关节炎的严重程度增加。关节软骨囊泡（ACV）在ATP和BCP晶体与添加的β-甘油磷酸盐的存在下产生CPPD晶体。虽然ACV存在于正常的关节软骨中，但它们主要在骨关节炎关节的软骨中矿化。本研究的目的是探索ACV矿化受周围细胞外基质成分调节的假设。将猪ACV包埋在含有II型和/或I型胶原和/或蛋白聚糖的琼脂糖凝胶中。矿化作用被测量为ATP或β-甘油磷酸盐刺激的45 Ca积累，并反映成核和生长。将合成的CPPD和BCP晶体包埋在类似的凝胶中以分离基质组分对晶体生长的影响。在琼脂糖凝胶中建立ACV对ATP和β-甘油磷酸盐的基线反应性后，我们检查了ATP和β-甘油磷酸盐刺激含有各种基质组分的凝胶中矿物质形成的能力。II型胶原抑制ATP刺激矿化的能力，而II型胶原加I型胶原的组合增加ATP和β -甘油磷酸盐对矿化的作用。I型胶原以剂量反应方式影响ACV矿化。两种类型的胶原蛋白都没有显著影响晶体生长或矿化调节酶的水平。蛋白聚糖抑制矿物形成ACV在凝胶中含有I型和II型胶原蛋白。骨关节炎发生的软骨基质变化，如I型胶原含量增加和蛋白多糖水平降低，可能促进ACV矿化。
Calcium pyrophosphate dihydrate (CPPD) and basic calcium phosphate (BCP) crystals occur in up to 60% of osteoarthritic joints and predict an increased severity of arthritis. Articular cartilage vesicles (ACVs) generate CPPD crystals in the presence of ATP and BCP crystals with added β-glycerophosphate. While ACVs are present in normal articular cartilage, they mineralize primarily in cartilage from osteoarthritic joints. The aim of this study was to explore the hypothesis that ACV mineralization is regulated by components of the surrounding extracellular matrix. Porcine ACVs were embedded in aga-rose gels containing type II and/or type I collagen and/or proteoglycans. Mineralization was measured as45Ca accumulation stimulated by ATP or β-glycerophosphate and reflects both nucleation and growth. Synthetic CPPD and BCP crystals were embedded in similar gels to isolate the effect of matrix components on crystal growth. After establishing baseline responsiveness of ACVs to ATP and β-glycerophosphate in agarose gels, we examined the ability of ATP and β-glycerophosphate to stimulate mineral formation in gels containing various matrix components. Type II collagen suppressed the ability of ATP to stimulate mineralization, while a combination of type II plus type I collagen increased the effect of ATP and β -glycerophosphate on mineralization. Type I collagen affected ACV mineralization in a dose-responsive manner. Neither type of collagen significantly affected crystal growth or levels of mineralization-regulating enzymes. Proteoglycans suppressed mineral formation by ACVs in gels containing both type I and type II collagen. Cartilage matrix changes that occur with osteoarthritis, such as increased quantities of type I collagen and reduced proteoglycan levels, may promote ACV mineralization.
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影响因子：
4.2
作者：
Derfus, B;Kranendonk, S;Ryan, L
通讯作者：
Ryan, L
影响因子：
7
作者：
Derfus, BA;Camacho, NP;Rosenthal, AK
通讯作者：
Rosenthal, AK
影响因子：
4.2
作者：
CHEN, CC;BOSKEY, AL
通讯作者：
BOSKEY, AL
影响因子：
4.2
作者：
BOSKEY, AL;CAMACHO, NP;BINDERMAN, I
通讯作者：
BINDERMAN, I
影响因子：
--
作者：
DERFUS, BA;RACHOW, JW;RYAN, LM
通讯作者：
RYAN, LM