Effects of Environmental Factors on Anammox Bacterial Community Structure in Sediments of a Freshwater Aquaculture Farm, Yangcheng Lake
Effects of Environmental Factors on Anammox Bacterial Community Structure in Sediments of a Freshwater Aquaculture Farm, Yangcheng Lake
复制标题
环境因素对阳澄湖淡水养殖场沉积物厌氧氨氧化细菌群落结构的影响
DOI：
10.1080/01490451.2015.1054005
复制
发表时间：
2016-02
影响因子：
2.3
通讯作者：
Li Xiaowan
中科院分区：
文献类型：
--
作者：
Zhang Yaping;Ruan Xiaohong;Wan Yu;Li Xiaowan
摘要 先前的研究报道了厌氧氨氧化（anammox）细菌在各种生态系统中的广泛分布。然而，人们对集约化水产养殖系统中不同环境条件下厌氧氨氧化细菌的分布知之甚少。在长三角著名蟹场阳澄湖，于10月（摄食期）和1月（非摄食期）采集沉积物样品，分析厌氧氨氧化菌的分布、多样性及其与环境因子的关系。基于厌氧氨氧化菌功能生物标志物hzo基因，构建厌氧氨氧化菌克隆文库，并通过定量PCR（qPCR）测定其丰度。在湖中检测到厌氧氨氧化细菌，丰度范围为每克沉积物 0.70 × 105 至 6.05 × 105 个拷贝。来自 8 个克隆文库的序列产生了 7 个独特的操作分类单元 (OTU)，它们与 Candidatus Jettenia 属有远亲关系，相似度约为 91%。厌氧氨氧化细菌群落结构、多样性和丰度随环境条件而时空变化。 10月份氮化合物水平、厌氧氨氧化菌多样性、均匀度和丰度均高于1月份。样本的主要 OTU 从 1 月份的 HZO-OTU-1 (34.25%) 变为 10 月份的 HZO-OTU-2 (28.90%)。此外，靠近污水入口的站点（SW）在一月份缺乏HZO-OTU-7。典型对应分析（CCA）表明，孔隙水NO2−浓度、铵与氮氧化物比（NH4+/NOx−）和总有机碳与总氮比（TOC/TN）对厌氧氨氧化细菌群落结构差异影响最大。 Pearson相关分析表明，Anammox细菌丰度与孔隙水中TN、NH4+、NO3−浓度呈正相关，与孔隙水中TOC/TN呈负相关。
ABSTRACT Previous studies have reported wide distribution of anaerobic ammonia oxidation (anammox) bacteria in various ecosystems. However, little is known about the distribution of anammox bacteria under varying environmental conditions in intensive aquaculture systems. In Yangcheng Lake, a famous crab farm situated in the Yangtze River Delta, sediment samples were collected in October (feeding period) and January (nonfeeding period) to analyze the distribution and diversity of anammox bacteria and their relationships with environmental factors. Based on the functional biomarker of Anammox bacteria, hzo gene, anammox bacterial clone libraries were constructed and their abundances were determined by quantitative PCR (qPCR). The Anammox bacteria were detected in the lake with the abundances ranging from 0.70 × 105 to 6.05 × 105 copies per gram of sediment. Sequences from eight clone libraries yielded seven unique operational taxonomic units (OTUs), distantly related to the Candidatus Jettenia genera with a similarity of about 91%. The Anammox bacterial community structures, diversities and abundances varied spatiotemporally with environmental conditions. In October, the level of the nitrogen compounds, the diversity, evenness and abundance of Anammox bacteria were higher than in January. The predominant OTU of samples changed from HZO-OTU-1 (34.25%) in January to HZO-OTU-2 (28.90%) in October. Moreover, the site (SW) nearing to sewage inlet was lack of HZO-OTU-7 in January. Canonical correspondence analysis (CCA) showed that the pore water NO2− concentration, ammonium to nitrogen oxides ratio (NH4+/NOx−) and total organic carbon to total nitrogen ratio (TOC/TN) contributed most to Anammox bacterial community structures variances. Pearson correlations analysis revealed that the Anammox bacteria abundance had positive co-relationships with TN, NH4+, NO3− concentrations, and negative correlation with TOC/TN in porewater.
登录
查看更多内容
影响因子：
4.4
作者：
M. Strous;J. Kuenen;M. Jetten
通讯作者：
M. Strous;J. Kuenen;M. Jetten
DOI：
--
发表时间：
2012
期刊：
--
影响因子：
--
作者：
Ruan Xiao-hong
通讯作者：
Ruan Xiao-hong
影响因子：
4.4
作者：
Shimamura, Munetaka;Nishiyama, Takashi;Fujii, Takao
通讯作者：
Fujii, Takao
影响因子：
64.8
作者：
Kuypers, MMM;Sliekers, AO;Jetten, MSM
通讯作者：
Jetten, MSM
影响因子：
5
作者：
Li, Meng;Gu, Ji-Dong
通讯作者：
Gu, Ji-Dong