Gene expression analysis of conventional and interactive human gingival cell systems exposed to dental composites.
Gene expression analysis of conventional and interactive human gingival cell systems exposed to dental composites.
复制标题
暴露于牙科复合材料的常规和交互式人类牙龈细胞系统的基因表达分析
DOI：
10.1016/j.dental.2015.08.157
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Polydorou O
中科院分区：
文献类型：
--
作者：
Schulz SD;Rüppell C;Tomakidi P;Steinberg T;Reichl FX;Hellwig E;Polydorou O
本研究的目的是检测牙科复合材料在常规和交互牙龈细胞系统中与基因表达相关的作用。方法按照ISO10993-12：2012制造的不同复合材料的洗脱液(Ceram X®、Filtek™超级XT、Filtek™Silorane、Fusio™Liquid Dinn和Vertise™Flow)，分别在由人牙龈成纤维细胞和永生化的人牙龈角质形成细胞(IGK)组成的常规单一培养和交互细胞系统中暴露24小时和7天。采用定量逆转录-聚合酶链式反应分析细胞增殖、分化、凋亡、炎症和黏附的生物标志物。结果由于核糖核酸含量低，Vertise™Flow和Fusio™液态牙本质在培养7d时不能进行定量聚合酶链式反应。HGF培养显示两种细胞系的细胞黏附标志物转录水平均较低。在第7天，Filtek™SUPREST XT显示IHGK的两个细胞系统的微丝蛋白基因表达升高，从而促进分化，而Filtek™Silorane和Ceram X®在两种细胞类型中都产生了炎症生物标志物的升高。Annexin-V标记显示Flowables和Filtek™Supreme XT的IHGK凋亡率较高，而Filtek™Silorane和Ceram X®的凋亡率较低。
ObjectivesThe aim of this study was the detection of putative gene expression-related effects of dental composites in conventional and interactive gingival cell systems.MethodsConventional monoculture (MC) and interactive cell systems (ICS) comprising human gingival fibroblast (HGF) and immortalized human gingival keratinocytes (IHGK) were exposed for 24 h and 7 days according to ISO10993-12:2012 manufactured eluates of different composites (Ceram X®, Filtek™ Supreme XT, Filtek™ Silorane, Fusio™ Liquid Dentin, and Vertise™ Flow). qRT-PCR-based mRNA analysis for biomarkers indicating cell proliferation, differentiation, apoptosis, inflammation, and adhesion was performed. Apoptotic cells were quantified by annexin-V labeling.ResultsDue to low RNA amounts, qPCR could not be performed for Vertise™ Flow and Fusio™ Liquid Dentin at day 7. At 24 h, flowables yielded increased transcription for biomarkers of inflammation and apoptosis in IHGK, irrespective of the cell system. HGF cultures displayed lower transcription for cell adhesion markers in both cell systems. Filtek™ Supreme XT showed increased differentiation by elevated filaggrin gene expression in both cell systems for IHGK at day 7, while Filtek™ Silorane and Ceram X®yielded elevation of inflammation biomarkers in both cell types. Annexin-V labeling revealed high apoptosis rates for both flowables and Filtek™ Supreme XT for IHGK, while low rates were detected for Filtek™ Silorane and Ceram X®.SignificanceAmong the composites evaluated, exposition of IHGK and HGF in conventional and interactive cell systems demonstrated most pronounced gene expression alterations in response to flowables, coinciding with elevated levels of apoptosis.
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对两种用 LED 和卤素固化灯固化的复合树脂中洗脱的单体进行 HPLC 分析。
DOI：
--
发表时间：
2009
期刊：
Journal of Biomedical Materials Research. Part B - Applied biomaterials
影响因子：
--
作者：
M. Tabatabaee;Hosein Mahdavi;S. Zandi;M. Kharrazi
通讯作者：
M. Kharrazi
影响因子：
1.2
作者：
Ilday NO;Celik N;Dilsiz A;Alp HH;Aydin T;Seven N;Kiziltunç A
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Kiziltunç A
影响因子：
4.4
作者：
Tong, Louis;Corrales, Rosa M.;Pflugfelder, Stephen C.
通讯作者：
Pflugfelder, Stephen C.
影响因子：
3.4
作者：
Al-Hiyasat, AS;Darmani, H;Milhem, MM
通讯作者：
Milhem, MM
含树脂修复材料在人工唾液中老化后的体外细胞毒性
影响因子：
3.4
作者：
J. Wataha;F. Rueggeberg;C. Lapp;J. Lewis;P. Lockwood;J. W. Ergle;D. Mettenburg
通讯作者：
D. Mettenburg