In vitro cytotoxicity of resin-containing restorative materials after aging in artificial saliva
In vitro cytotoxicity of resin-containing restorative materials after aging in artificial saliva
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含树脂修复材料在人工唾液中老化后的体外细胞毒性
DOI：
10.1007/s007840050093
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发表时间：
1999
影响因子：
3.4
通讯作者：
D. Mettenburg
中科院分区：
文献类型：
--
作者：
J. Wataha;F. Rueggeberg;C. Lapp;J. Lewis;P. Lockwood;J. W. Ergle;D. Mettenburg
摘要研究表明，牙科树脂基材料会释放出具有生物学责任的物质。 然而，目前用于检测这些物质的一些方法可能不足以检测生物相关浓度。在本研究中，我们假设当高压液相色谱法（HPLC-UV检测）无法检测到任何物质释放时，树脂基材料在体外表现出细胞毒性效应并改变细胞功能。我们进一步假设，即使在人工唾液中老化样品后，这种释放仍在继续。在人工唾液中老化0、7或14天后，对5种类型的复合材料或复合体材料（Z-100、Tetric Ceram、Dyract AP、Solitaire和Clearfil AP-X）和一种有机改性陶瓷材料（Definite）进行了测试。通过与成纤维细胞直接接触并在老化后暴露48小时后测量琥珀酸脱氢酶活性来评估细胞毒性。使用HPLC和UV检测评估物质从材料中的释放。通过用磺酰罗丹明染色测量MCF-7细胞的增殖来估计改变的细胞功能。HPLC显示，尽管在未老化的情况下物质的初始释放较高，但在老化7或14天后该释放显著下降，并且对于四种材料（Tetric Ceram、Definite、Solitaire和Clearfil AP-X），在14天后与Teflon对照品相当。在没有唾液老化的情况下，所有材料的细胞毒性> Teflon阴性对照的50%。老化14天后，除Definite外，所有材料均继续显示出严重的细胞毒性。由于其他材料的持续毒性，只能测试Definite改变细胞功能的能力。这种改性的陶瓷材料对MCF-7细胞产生显著的增殖作用，表明释放了足够的物质来改变细胞功能。我们的结论是，所有这些市售的树脂基牙科材料即使在人工唾液中老化2周后，仍会继续释放足够的成分，导致体外致死效应或改变细胞功能。
Abstract Studies have reported that dental resin-based materials release substances which have biological liabilities. However, some current methods for detecting these substances may not be adequate to detect biologically relevant concentrations. In the current study, we hypothesized that resin-based materials exhibit cytotoxic effects and alter cellular function in vitro when high-pressure liquid chromatography (HPLC-UV detection) cannot detect any release of substances. We further hypothesized that this release continues even after aging the samples in artificial saliva. Five types of composite or compomer materials (Z-100, Tetric Ceram, Dyract AP, Solitaire, and Clearfil AP-X) and one organically modified ceramic material (Definite) were tested after aging in artificial saliva for 0, 7, or 14 days. Cytotoxicity was assessed using direct contact with fibroblasts and measurement of succinic dehydrogenase activity after 48 h of exposure post aging. Release of substances from the materials was assessed using HPLC with UV detection. Altered cellular function was estimated by measuring proliferation of MCF-7 cells with sulforhodamine staining. HPLC showed that whereas initial release of substances was higher without aging, this release dropped significantly after 7 or 14 days of aging, and was equivalent to the Teflon controls after 14 days for four of the materials (Tetric Ceram, Definite, Solitaire, and Clearfil AP-X). Without aging in saliva, all materials had cytotoxicities >50% of the Teflon negative controls. After 14 days of aging, all materials except the Definite continued to show severe cytotoxicity. Only the Definite could be tested for its ability to alter cellular function because of the continuing toxicity of the other materials. This modified ceramic material caused a significant proliferative effect on the MCF-7 cells indicating that sufficient substances were released to alter cellular function. We concluded that all of these commercially available resin-based dental materials continue to release sufficient components to cause lethal effects or alter cellular function in vitro even after 2 weeks of aging in artificial saliva.
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影响因子：
10.4
作者：
Olea N;Pulgar R;Pérez P;Olea-Serrano F;Rivas A;Novillo-Fertrell A;Pedraza V;Soto AM;Sonnenschein C
通讯作者：
Sonnenschein C
影响因子：
4.2
作者：
Bouillaguet, S;Wataha, JC;Holz, J
通讯作者：
Holz, J
体外合金细胞毒性测试的精度和新方法。
DOI：
10.1016/0109-5641(92)90056-i
发表时间：
1992
期刊：
Dental materials : official publication of the Academy of Dental Materials
影响因子：
--
作者：
Wataha,JC;Craig,RG;Hanks,CT
通讯作者：
Hanks,CT
金属阳离子对真核细胞代谢的体外影响。
DOI：
10.1002/jbm.820250907
发表时间：
1991
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
Wataha,JC;Hanks,CT;Craig,RG
通讯作者：
Craig,RG
影响因子：
2.9
作者：
HANKS, CT;WATAHA, JC;FAT, JC
通讯作者：
FAT, JC