The native Wolbachia endosymbionts of Drosophila melanogaster and Culex quinquefasciatus increase host resistance to West Nile virus infection.
The native Wolbachia endosymbionts of Drosophila melanogaster and Culex quinquefasciatus increase host resistance to West Nile virus infection.
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DOI：
10.1371/journal.pone.0011977
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发表时间：
2010-08-05
期刊：
影响因子：
3.7
通讯作者：
Meola MA
中科院分区：
文献类型：
--
作者：
Glaser RL;Meola MA
已显示细菌内共生体Wolbachia pipientis在天然果蝇宿主中和在通常无Wolbachia的异源宿主埃及伊蚊中当被来自黑腹果蝇或白纹伊蚊的Wolbachia感染时增加宿主对病毒感染的抗性。沃尔巴克氏体感染尚未被证明在天然沃尔巴克氏体-蚊子宿主系统中增加病毒抗性。在这项研究中，我们研究了沃尔巴克氏体诱导的抵抗西尼罗河病毒（WNV;黄病毒科）的感染敏感性，在沃尔巴克氏体感染和无沃尔巴克氏体的D。黑腹库蚊和致倦库蚊（Culex quinquefasciatus），一种西尼罗河病毒的天然蚊媒。Wolbachia感染D. melanogaster诱导对WNV感染的强抗性。与缺乏沃尔巴克氏体的果蝇相比，沃尔巴克氏体感染的果蝇对WNV的ID 50高500倍，产生的病毒滴度低100，000倍。抗性表型作为母体细胞质因子传播，并在沃尔巴克氏体治愈的果蝇中完全恢复。Wolbachia感染对D.黑腹病毒到基孔肯雅病毒（披膜病毒科）和拉克罗斯病毒（布尼亚病毒科）。Wolbachia还诱导Cx对WNV感染的抗性。致倦肌虽然沃尔巴克氏体对西尼罗河病毒经口感染的总体速率没有影响，但与缺乏沃尔巴克氏体的蚊子相比，沃尔巴克氏体感染的蚊子产生的病毒滴度较低，病毒传播率低2至3倍。这是第一次证明沃尔巴克氏体可以增加对虫媒病毒感染的抵抗力，从而减少本地沃尔巴克氏体-蚊子系统中的病毒传播。结果表明，沃尔巴克氏体降低Cx中的载体能力。致倦犬，并可能在其他沃尔巴克氏体感染的蚊子载体。
The bacterial endosymbiont Wolbachia pipientis has been shown to increase host resistance to viral infection in native Drosophila hosts and in the normally Wolbachia-free heterologous host Aedes aegypti when infected by Wolbachia from Drosophila melanogaster or Aedes albopictus. Wolbachia infection has not yet been demonstrated to increase viral resistance in a native Wolbachia-mosquito host system. In this study, we investigated Wolbachia-induced resistance to West Nile virus (WNV; Flaviviridae) by measuring infection susceptibility in Wolbachia-infected and Wolbachia-free D. melanogaster and Culex quinquefasciatus, a natural mosquito vector of WNV. Wolbachia infection of D. melanogaster induces strong resistance to WNV infection. Wolbachia-infected flies had a 500-fold higher ID50 for WNV and produced 100,000-fold lower virus titers compared to flies lacking Wolbachia. The resistance phenotype was transmitted as a maternal, cytoplasmic factor and was fully reverted in flies cured of Wolbachia. Wolbachia infection had much less effect on the susceptibility of D. melanogaster to Chikungunya (Togaviridae) and La Crosse (Bunyaviridae) viruses. Wolbachia also induces resistance to WNV infection in Cx. quinquefasciatus. While Wolbachia had no effect on the overall rate of peroral infection by WNV, Wolbachia-infected mosquitoes produced lower virus titers and had 2 to 3-fold lower rates of virus transmission compared to mosquitoes lacking Wolbachia. This is the first demonstration that Wolbachia can increase resistance to arbovirus infection resulting in decreased virus transmission in a native Wolbachia-mosquito system. The results suggest that Wolbachia reduces vector competence in Cx. quinquefasciatus, and potentially in other Wolbachia-infected mosquito vectors.
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